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Molecular size markers

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Molecular size markers are laboratory tools used to determine the molecular weight or size of biomolecules, such as proteins, nucleic acids, or polysaccharides, in electrophoresis experiments. They provide a reference scale against which the size of the target molecules can be compared.

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Lab products found in correlation

Digidoc it, by Analytik Jena (1 mentions) Agarose gel, by Thermo Fisher Scientific (1 mentions) Molecular biological enzymes, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Culture media, by Thermo Fisher Scientific (1 mentions)

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Size Marker (2 citations)

2 protocols using molecular size markers

1

RAPD Amplification and Gel Electrophoresis

Cited 1 time
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The RAPD amplification mixture and cycling conditions were described elsewhere (Lambiase et al., 2006 (link)). The primer used was the 270 (5′-TGCGCGCGGG-3′). RAPD products were separated by electrophoresis in 1.5% agarose gel (Invitrogen Corporation, CA). Molecular size markers (Invitrogen Corporation) and negative control were included in all gels. Gels were stained with EtBr (at 0.5 μM) (Invitrogen Corporation) and captured with a DigiDoc-It (UVP) photographic system.
Trancassini M., Iebba V., Citerà N., Tuccio V., Magni A., Varesi P., De Biase R.V., Totino V., Santangelo F., Gagliardi A, & Schippa S. (2014). Outbreak of Achromobacter xylosoxidans in an Italian Cystic fibrosis center: genome variability, biofilm production, antibiotic resistance, and motility in isolated strains. Frontiers in Microbiology, 5, 138.
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2

TIMP-3 Protein Assay Protocol

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Unless otherwise noted, all chemicals and reagents were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO). Molecular biological enzymes, molecular size markers, culture media, and Trizol were purchased from Invitrogen Life Technologies, Inc. (Carlsbad, CA). Oligonucleotide primers were ordered from the Integrated DNA Technologies, Inc. Recombinant TIMP-3 Protein (Cat #: 973-TM-010) was purchased from the R&D Systems company, and 0.1µg/ml was used for this study.
Pan B., Zhan X, & Li J. (2022). MicroRNA-574 Impacts Granulosa Cell Estradiol Production via Targeting TIMP3 and ERK1/2 Signaling Pathway. Frontiers in Endocrinology, 13, 852127.
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