Rabbit il 10

Manufactured by Abcam

Rabbit IL-10 is a recombinant protein that corresponds to the cytokine interleukin-10 from the rabbit. Interleukin-10 is an anti-inflammatory cytokine that regulates the immune response.

Automatically generated - may contain errors

Lab products found in correlation

Bicinchoninic acid assay, by Thermo Fisher Scientific (4 mentions) Il 12, by Abcam (2 mentions) Rabbit p erk, by Cell Signaling Technology (2 mentions) Rabbit p akt, by Abcam (2 mentions) Rabbit jnk, by Abcam (2 mentions) Rabbit p jnk, by Abcam (2 mentions) Rabbit erk, by Cell Signaling Technology (2 mentions) Rabbit β tubulin, by Merck Group (2 mentions) Rabbit akt, by Abcam (1 mentions) Sds polyacrylamide gel electrophoresis, by Thermo Fisher Scientific (1 mentions) Sds page, by Thermo Fisher Scientific (1 mentions)

2 protocols using rabbit il 10

Characterization of Macrophage and Smooth Muscle Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peritoneal macrophages, RAW 264.7 and hASMCs were homogenized in RIPA lysis buffer. Protein concentrations were determined through the Bicinchoninic Acid assay (Thermo Scientific) according to the manufacturer’s protocol. SDS–polyacrylamide gel electrophoresis (Invitrogen) was performed using 10 μg of protein per well. Western blot was performed with the following primary antibodies: rabbit Chi3l1 (dilution 1:1,000), rabbit Akt (1:500), rabbit p-Akt (1:500), rabbit JNK (1:3,000), rabbit p-JNK (1:3,000) and rabbit IL-10 (1:1,000; all obtained from Abcam), and IL-12 (1:1,000), rabbit ERK (1:3,000) and rabbit p-ERK (1:3,000; all obtained from Cell Signaling), and rabbit β-tubulin (1:1,000; obtained from Sigma-Aldrich; Supplementary Fig. 10).

Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.

+ Open protocol

+ Expand

Protein Expression and Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peritoneal macrophages, RAW 264.7, and hASMCs were homogenized in RIPA lysis buffer. Protein concentrations were determined through the Bicinchoninic Acid assay (ThermoScientific), according to the manufacturer's protocol. SDS-PAGE (Invitrogen) was performed using 10µg of protein per well. Western blot was performed with the following primary antibodies: rabbit Chi3l1 (dilution 1:1000), rabbit Akt (1:500), rabbit p-Akt (1:500), rabbit JNK (1:3000), rabbit p-JNK (1:3000), rabbit IL-10 (1:1000; all from Abcam) and IL-12 (1:1000), rabbit ERK (1:3000), rabbit p-ERK (1:3000; all from Cell Signaling) and rabbit β-tubulin (1: 1000; both Sigma Aldrich; Supplementary Fig. 10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!