Apc conjugated anti cxcr4 antibody

Manufactured by Abcam

Sourced in United States

The APC-conjugated anti-CXCR4 antibody is a fluorescently labeled antibody that binds to the CXCR4 chemokine receptor. CXCR4 is a G-protein coupled receptor that plays a role in cell migration and signaling.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (2 mentions) Cell dissociation buffer, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

CXCR4 (91 citations) Anti-CXCR4 (58 citations) Anti-CXCR4 antibody (23 citations) CXCR4 antibody (6 citations) Anti-APC antibody (2 citations)

2 protocols using apc conjugated anti cxcr4 antibody

CXCR4 Expression in Mz-ChA-1 Cells

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Mz-ChA-1 cells were detached with enzyme-free Cell Dissociation Buffer (Thermo Scientific) and suspended in a staining buffer. Cells were stained live with allophycocyanin (APC)-conjugated anti- CXCR4 antibody (Abcam, USA) for 1 h at 4 °C. Isotype-matched negative control was used in the panel of mAb to assess background fluorescence intensity. Samples were analyzed on a BD FACS Calibur flow cytometer (BD Bioscience, Bedford, MA). The results were processed using FlowJo software (Tree Star Inc., Ashland, OR).

Xie Y., Wang Y., Li J., Hang Y., Jaramillo L., Wehrkamp C.J., Phillippi M.A., Mohr A.M., Chen Y., Talmon G.A., Mott J.L, & Oupický D. (2018). Cholangiocarcinoma therapy with nanoparticles that combine downregulation of MicroRNA-210 with inhibition of cancer cell invasiveness. Theranostics, 8(16), 4305-4320.

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CXCR4 expression in HuCCT1 cells

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HuCCT1 cells were detached with enzyme-free Cell Dissociation Buffer (Thermo Scientific) and suspended in a staining buffer. Cells were stained live with allophycocyanin (APC)-conjugated anti-CXCR4 antibody (Abcam, USA) for 1 h at 4 °C. Isotype-matched negative control was used in the panel of mAb to assess background fluorescence intensity. Samples were analyzed on a BD FACSCalibur flow cytometer (BD Bioscience, Bedford, MA). The results were processed using FlowJo software (Tree Star Inc., Ashland, OR).

Xie Y., Wehrkamp C.J., Li J., Wang Y., Wang Y., Mott J.L, & Oupický D. (2016). Delivery of miR-200c Mimic with Poly(amido amine) CXCR4 Antagonists for Combined Inhibition of Cholangiocarcinoma Cell Invasiveness. Molecular pharmaceutics, 13(3), 1073-1080.

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