Recombinant αKlotho protein containing the ectodomain of mouse αKlotho (amino acid number 31–982) with C-terminal V5 and 6xHis tags was generated in our laboratory as described (39 (link), 40 (link)). For the in vivo experiments, recombinant αKlotho protein (0.3 mg/kg body weight) or vehicle (normal saline) was given for 4 weeks via ALZET® 1004 osmotic minipumps (DURECT Corporation, Cupertino, CA).
Alzet 1004 osmotic minipumps
Manufactured by Durect
The ALZET® 1004 osmotic minipump is a laboratory device designed for continuous and controlled delivery of test substances to small laboratory animals. It functions by using the principle of osmosis to deliver the substance at a pre-determined release rate over an extended period of time.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alzet 1004 osmotic minipumps
1
Recombinant αKlotho Protein Administration
2
Modulating Autophagy in Mice
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