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Ct bkm120

Manufactured by Chemietek
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The CT-BKM120 is a laboratory instrument designed for the analysis and measurement of chemical samples. It is capable of performing a variety of analytical techniques, but its core function is to provide accurate and reliable data for research and testing purposes.

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Lab products found in correlation

D8418, by Merck Group (1 mentions) Api 2, by Bio-Techne (1 mentions) Paclitaxel, by Selleck Chemicals (1 mentions) Bkm120, by Chemietek (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

3 protocols using ct bkm120

1

Evaluation of Tankyrase, PI3K, and AKT Inhibitors

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All small-molecule inhibitors were dissolved in DMSO (D8418, Sigma-Aldrich) and kept as 10 mM stocks at 4 oC. General protocol for treatment: Cells were seeded one day before treatment to reach ∼20 or ∼80% confluence for a 72 hour or a 24 hour treatment, respectively. The medium was changed to medium containing vehicle (0.01% DMSO), 1 μM or various doses of the tankyrase inhibitor G007-LK (Mercachem), PI3K inhibitor BKM120 (CT-BKM120, Chemietek) or AKT inhibitor API-2 (2151, Tocris Bioscience).
Mygland L., Brinch S.A., Strand M.F., Olsen P.A., Aizenshtadt A., Lund K., Solberg N.T., Lycke M., Thorvaldsen T.E., Espada S., Misaghian D., Page C.M., Agafonov O., Nygård S., Chi N.W., Lin E., Tan J., Yu Y., Costa M., Krauss S, & Waaler J. (2021). Identification of response signatures for tankyrase inhibitor treatment in tumor cell lines. iScience, 24(7), 102807.
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2

Preparation of Anticancer Drug Solutions

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BKM120 (Catalog no. CT-BKM120) was purchased from Chemietek. Eribulin was provided by Eisai. Paclitaxel (Catalog no. S1150) was purchased from Selleckchem. All drugs were prepared in stock solution of 10 mM in dimethyl sulfoxide (DMSO; Sigma) for in vitro experiments.
Rajput S., Guo Z., Li S, & Ma C.X. (2019). PI3K inhibition enhances the anti-tumor effect of eribulin in triple negative breast cancer. Oncotarget, 10(38), 3667-3680.
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3

Evaluating BKM120 and PTEN IHC

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BKM120 (Cat. No. CT-BKM120) was purchased from Chemietek. The primary antibody for PTEN IHC was purchased from Cell Signaling Technology (Cat. No. 9559).
Guo Z., Primeau T., Luo J., Zhang C., Sun H., Hoog J., Gao F., Huang S., Edwards D.P., Davies S.R., Aft R., Ding L., Ellis M.J., Li S, & Ma C.X. (2020). Proteomic Resistance Biomarkers for PI3K Inhibitor in Triple Negative Breast Cancer Patient-Derived Xenograft Models. Cancers, 12(12), 3857.
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