Goat anti mouse igg1

Manufactured by Abcam

Sourced in United Kingdom

Goat anti-mouse IgG1 is a secondary antibody that binds specifically to the mouse IgG1 isotype. It can be used in various immunoassay techniques to detect and quantify mouse IgG1 proteins.

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Lab products found in correlation

Rabbit anti monkey igg hrp, by Bioss Antibodies (2 mentions) Goat anti human igg hrp conjugate, by Merck Group (2 mentions) Microtiter plate, by Corning (2 mentions) Goat anti mouse igg2a, by Abcam (2 mentions) Elisa reader, by Tecan (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions) Goat anti mouse igg3, by Abcam (1 mentions) 3 3 5 5 tetramethylbenzidine tmb substrate, by Merck Group (1 mentions) Spark reader, by Tecan (1 mentions) Hrp conjugated goat anti mouse igg, by Beyotime (1 mentions) 5 5 tetramethylbenzidine tmb, by Beyotime (1 mentions) Infinite m200 pro microplate reader, by Tecan (1 mentions)

5 protocols using goat anti mouse igg1

SARS-CoV-2 Antibody Detection by ELISA

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The microtiter plates (Corning, USA) were coated overnight with 2 μg/ml of SARS-CoV-2 S (Sino Biological, 40589-V08B1) or RBD proteins (Sino Biological, 40592-V08H). Serum samples were twofold serially diluted and S or RBD binding antibody (S-BAb or RBD-BAb) were detected by ELISA. Secondary antibodies were goat anti-mouse IgG-HRP (Beijing Bersee Science and Technology, Co. Ltd, China), rabbit anti-monkey IgG-HRP (Bioss, China) and goat anti-human IgG-HRP conjugates (Sigma, USA), respectively. Endpoint titers were defined as the highest reciprocal serum dilution that yielded an absorbance >0.2 and a ratio of signal than cutoff (S/CO) >1. Log10 end point titers were reported [24 (link)].
Goat anti-mouse IgG1, IgG2a, IgG2b, or IgG3 heavy chain-HRP conjugates were used for IgG sub-classification according to manufacturer's instruction (Abcam, UK).

Luo S., Zhang P., Liu B., Yang C., Liang C., Wang Q., Zhang L., Tang X., Li J., Hou S., Zeng J., Fu Y., Allain J.P., Li T., Zhang Y, & Li C. (2021). Prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates. Emerging Microbes & Infections, 10(1), 1002-1015.

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OVA-Specific Antibody ELISA Protocol

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A 96-well plate was precoated with 200 μg OVA in 0.1 M NaHCO₃ at pH 9.6 of coating solution at 4 °C overnight. After PBS washes and blocking with 1% BSA (Sigma-Aldrich Co.), sera were loaded into wells and incubated at 4 °C overnight. After PBS washes, the wells were incubated with horseradish peroxidase (HRP)-conjugated rat anti-mouse IgE (GeneTex, Inc., Hsinchu, Taiwan) or goat anti-mouse IgG1 (Abcam, Cambridge, UK) antibodies at 4 °C overnight. After PBS washes, 3,3′,5,5′-tetramethylbenzidine (TMB) (Sigma) was added to the wells and incubated at room temperature for 15 min. The absorbance at 450 nm was measured using an ELISA reader (Tecan Trading AG, Männedorf, Switzerland).

Lin C.C., Chuang K.C., Chen S.W., Chao Y.H., Yen C.C., Yang S.H., Chen W., Chang K.H., Chang Y.K, & Chen C.M. (2022). Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses. International Journal of Molecular Sciences, 23(22), 14185.

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Chikungunya Virus IgG Isotype ELISA

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ELISA Nunc-Immuno 96-well MaxiSorp ELISA plates (Thermo Fisher Scientific, Darmstadt, Germany) were coated with 10⁶ PFUs CHIKV per well in 100 μL PBS at 4°C overnight. After blocking for 1 h with 1% BSA in PBS, serum samples were serially diluted (1:50, 1:250, 1:1,000, and 1:5,000) for IgG endpoint titer determination, or for isotype determination, serum samples were diluted 1:100 in PBS containing 0.05% Tween 20 and 1% BSA, and incubated for 1 h at 37°C. The following anti-mouse secondary HRP-coupled antibodies were then added for 1 h at room temperature: rabbit anti-mouse total IgG (1:2,000; dianova, Hamburg, Germany; no. 115-035-003), goat-anti-mouse IgG1 (1:5,000; Abcam, Cambridge, UK; no. ab97240), goat-anti-mouse IgG2a (1:5,000; Abcam; no. ab97245), goat-anti-mouse IgG2b (1:5,000; Abcam; no. ab97250), or goat-anti-mouse IgG3 (1:5,000; Abcam; no. ab97260). For detection, 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Merck Millipore, Darmstadt, Germany) was added, and the reaction was stopped after 15 min with 1 M H₂SO₄. Absorbance was measured at 450 nm (reference wavelength 620 nm) with a Tecan spark reader (Tecan, Männedorf, Switzerland).

Schmidt C., Haefner E., Gerbeth J., Beissert T., Sahin U., Perkovic M, & Schnierle B.S. (2022). A taRNA vaccine candidate induces a specific immune response that protects mice against Chikungunya virus infections. Molecular Therapy. Nucleic Acids, 28, 743-754.

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Quantifying SARS-CoV-2 RBD-specific Antibodies

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Enzyme-linked immunosorbent assay (ELISA) was used to quantify the SARS-CoV-2 RBD-specific IgG, IgG1 and IgG2a titers in serum based on a previous report [15 (link)], with minor modification. Briefly, ELISA plates were pre-coated with 2 µg/mL RBD of wildtype SARS-CoV-2 overnight at 4 °C. The coated plates were washed with phosphate-buffered saline (PBS) containing 0.05% Tween (PBS-T) and blocked with PBS containing 2.5% bovine serum albumin for 2 h at 37 °C. Mouse sera were serially diluted in PBS-T, followed by incubation of plates at 37 °C for 1 h. After four washes with PBS-T, HRP-conjugated goat anti-mouse IgG (Beyotime, Shanghai, China), goat anti-mouse IgG1 (abcam, Shanghai, China) or goat anti-mouse IgG2a (abcam, Shanghai, China) was applied to plates and incubated at 37 °C for 1 h. After the plates were washed thoroughly, 3, 3′, 5, 5′-Tetramethylbenzidine (TMB) (Beyotime, Shanghai, China) was added into the plates to visualize the reaction. Finally, 1 N H₂SO₄ was added into the plates to stop the reaction, and the absorbance value was collected at 450 nm using a Tecan Infinite M200PRO microplate reader (Männedorf, Switzerland). The endpoint titers were expressed as the highest reciprocal serum dilution exhibiting an absorbance of 450 nm > 2.1-fold over the background values.

Zhang N., Ji Q., Liu Z., Tang K., Xie Y., Li K., Zhou J., Li S., Shang H., Shi Z., Zheng T., Yao J., Lu L., Yuan S, & Jiang S. (2022). Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant. Viruses, 14(3), 501.

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SARS-CoV-2 Antibody Binding Assay

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The microtiter plates (Corning, USA) were coated overnight with 2μg/ml of SARS-CoV S or RBD proteins (Sino Biological, China). Serum samples were 2-fold serially diluted and S or RBD binding antibody (S-BAb or RBD-BAb) were detected by ELISA. Secondary antibodies were goat anti-mouse IgG-HRP (Beijing Bersee Science and Technology, Co. LTd, China), rabbit anti-monkey IgG-HRP (Bioss, China) and goat anti-human IgG-HRP conjugates (Sigma, USA), respectively.
Endpoint titers were defined as the highest reciprocal serum dilution that yielded an absorbance > 0.2 and a ratio of signal than cutoff (S/CO) > 1. Log10 end point titers were reported (26).
Goat anti-mouse IgG1, IgG2a, IgG2b or IgG3 heavy chain-HRP conjugates were used for IgG sub-classification according to manufacturer's instruction (Abcam, UK).

Luo S., Zhang P., Liu B., Yang C., Liang C., Wang Q., Zhang L., Tang X., Li J., Hou S., Zeng J., Fu Y., Allain J., Li T., Zhang Y., & Li C. (2020). Prime-boost vaccination of mice and Rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates.

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