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Anti citrate synthase

Manufactured by Cell Signaling Technology
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Anti-Citrate synthase is a lab equipment product that can be used to detect and quantify the presence of citrate synthase, a key enzyme in the citric acid cycle. It functions by binding to citrate synthase and allowing for its identification and measurement in various biological samples.

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Lab products found in correlation

Nbp1 90654, by Cell Signaling Technology (2 mentions) Ab22048, by Abcam (1 mentions) Ab181153, by Abcam (1 mentions) Alexa flour 488 conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Axiovert 200 microscope, by Zeiss (1 mentions) Ab46545, by Abcam (1 mentions) Anti β actin, by Abcam (1 mentions) Anti bnip3, by Cell Signaling Technology (1 mentions) Anti nix, by Cell Signaling Technology (1 mentions) Anti bnip3, by Abcam (1 mentions) Anti lc3b, by Cell Signaling Technology (1 mentions) Anti ago2, by Cell Signaling Technology (1 mentions) Anti phospho acc ser79, by Cell Signaling Technology (1 mentions) Anti s6 ribosomal protein, by Cell Signaling Technology (1 mentions) Anti acc, by Cell Signaling Technology (1 mentions) Anti ulk1, by Cell Signaling Technology (1 mentions) Pgc 1α, by Santa Cruz Biotechnology (1 mentions) Anti ampkα, by Cell Signaling Technology (1 mentions) Anti ampkα1, by Abcam (1 mentions) Anti phospho ulk1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Citrate Synthase (6 citations)

4 protocols using anti citrate synthase

1

Immunofluorescence Staining of Brain Sections

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Paraformaldehyde fixed frozen brain sections or BMVECs grown on slides were subsequently washed with TBS followed by treatment with 0.2% Triton X-100 for 3 minutes. After washing, samples were blocked by 5% BSA for 1 hour at room temperature. Slides were then incubated with primary antibodies like anti-IREB2 (iron responsive element binding protein 2) (ab181153, Abcam), anti-4 hydroxynonenal (anti-4HNE) (ab46545, Abcam), anti-TLR4 (toll like receptor 4) (ab22048, Abcam) and anti-citrate synthase (14309, Cell Signaling) at a 1:100 dilution in 0.2% BSA at 4°C overnight. Cells were washed and incubated with AlexaFlour 488 conjugated secondary antibody (Jackson Immuno Research Laboratories, Inc., West Grove, PA) at a 1:400 dilution at room temperature for 1 hour. Negative control slides were incubated with 0.2% BSA in place of the primary antibody. Slides were imaged on Axiovert 200 microscope (Carl Zeiss MicroImaging, Thornwood, NY).
Abdul Y., Li W., Ward R., Abdelsaid M., Hafez S., Dong G., Jamil S., Wolf V., Johnson M.H., Fagan S.C, & Ergul A. (2020). Deferoxamine Treatment Prevents Post-Stroke Vasoregression and Neurovascular Unit Remodeling Leading to Improved Functional Outcomes in Type 2 Male Diabetic Rats: Role of Endothelial Ferroptosis. Translational stroke research, 12(4), 615-630.
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2

Western Blot Analysis of Mitophagy Regulators

Cited 1 time
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Western blotting was performed as previously described14 (link). Antibodies used in this study include: anti-PPTC7 (Novus Biologicals, catalog #NBP1-90654, 1:1000 dilution, 48 h incubation), anti-Citrate synthase (Cell Signaling Technologies, catalog #14309, 1:1000 dilution, overnight incubation), anti-beta actin (Abcam, catalog # ab170325, 1:1000 dilution, overnight incubation), anti-BNIP3 (rodent specific antibody, Cell Signaling Technologies, catalog #3769, 1:1000 dilution, 48 h incubation), anti-NIX (Cell Signaling Technologies, catalog #12396, 1:1000 dilution, overnight incubation), and anti-LC3B (Cell Signaling Technologies, catalog #3868, 1:1000 dilution, overnight incubation). For the immunoprecipitations, the following antibodies were used: anti-BNIP3 (Abcam, clone EPR4034, catalog #ab109362, WB 1:1000), anti-NIX (Cell Signaling Technology, clone D4R4B, catalog #12396, WB 1:1000); anti-FLAG (Sigma-Aldrich, catalog #SAB4301135, WB 1:1000), and anti-vinculin (SCBT, clone G-11; sc-55465, WB 1:1000).
Niemi N.M., Serrano L.R., Muehlbauer L.K., Balnis C.E., Wei L., Smith A.J., Kozul K.L., Forny M., Connor O.M., Rashan E.H., Shishkova E., Schueler K.L., Keller M.P., Attie A.D., Friedman J.R., Pagan J.K., Coon J.J, & Pagliarini D.J. (2023). PPTC7 maintains mitochondrial protein content by suppressing receptor-mediated mitophagy. Nature Communications, 14, 6431.
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3

Western Blotting of Mitochondrial Proteins

Cited 1 time
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Western blotting was performed as previously described (Niemi et al., 2019 (link)). Antibodies used in this study include: anti-Pptc7 (Novus Biologicals, catalog #NBP1-90654, 1:1000 dilution, 48 hour incubation), anti-Citrate synthase (Cell Signaling Technologies, catalog #14309, 1:1000 dilution, overnight incubation), anti-beta actin (Abcam, catalog # ab170325, 1:1000 dilution, overnight incubation), anti-Bnip3 (rodent specific antibody, Cell Signaling Technologies, catalog #3769, 1:1000 dilution, 48 hour incubation), and anti-Nix (Cell Signaling Technologies, catalog #12396, 1:1000 dilution, overnight incubation).
Niemi N.M., Serrano L.R., Muehlbauer L.K., Balnis C., Kozul K.L., Rashan E.H., Shishkova E., Schueler K.L., Keller M.P., Attie A.D., Pagan J., Coon J.J, & Pagliarini D.J. (2023). Pptc7 maintains mitochondrial protein content by suppressing receptor-mediated mitophagy. bioRxiv.
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4

Immunoblotting Analysis of Cellular Signaling

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All biochemical reagents were purchased from Sigma-Aldrich unless otherwise indicated. Antibodies against, JNK1 (SC-1648, 1:2500), Dicer (SC-30226, 1:2500), Akt (SC-8312, 1:2500), phospho-Akt (Ser473) (SC-7985-R, 1:2500), PGC-1α (SC-13067, 1:2500), β-actin (SC-130656, 1:5000), and β-tubulin (SC-9104, 1:5000) were from Santa Cruz Biotechnology. Anti-Acc (3662, 1:2500), anti-phospho-Acc (Ser79) (11818, 1:2500), Anti-AMPKα (5832, 1:2500), Anti-phospho-AMPKα (Thr172) (2535, 1:2500), anti-AMPKβ (4250, 1:2500), anti-phospho-AMPKβ (Ser108) (4181, 1:2500), anti-phospho-ULK1 (Ser555) (5869, 1:2500), anti-phospho-ULK1 (Ser317) (12753, 1:2500), anti-ULK1 (8054, 1:2500), anti-phospho-MFF (Ser146) (49281, 1:2500), anti-MFF (86668, 1:2500), anti-Ago2 (2897, 1:2500), anti-Ago1 (5053, 1:2500), anti-S6 Ribosomal Protein (2217, 1:2500) and anti-phospho-JNK (Thr183/Tyr185) (1:2500), anti-Albumin (4929, 1:2500), anti-Citrate Synthase (14309, 1:2500), Anti-AMPKα1 (2795, 1:2500) were purchased from Cell Signaling Technology. Anti-IRS1 (1:2500) and anti-phospho-IRS (Ser307) (07247, 1:2500) antibodies were purchased from Upstate Biotechnology. Anti-AMPKα1 (32047, 1:2500) was purchased from Abcam.
Zhang C., Seo J., Murakami K., Salem E.S., Bernhard E., Borra V.J., Choi K., Yuan C.L., Chan C.C., Chen X., Huang T., Weirauch M.T., Divanovic S., Qi N.R., Thomas H.E., Mercer C.A., Siomi H, & Nakamura T. (2018). Hepatic Ago2-mediated RNA silencing controls energy metabolism linked to AMPK activation and obesity-associated pathophysiology. Nature Communications, 9, 3658.
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