All drugs were prepared and stored as per manufacturer indications. In detail, Doxorubicin (Sigma Aldrich, St. Louis, MO), 12-O-tetradecanoylphorbol-13-acetate (TPA) (EMD Millipore, Temecula, CA), and GSK126 (AdooQ Bioscience, Irvine, CA) were resuspended in 100% DMSO and stored at −20°C. Vincristine (AdipoGen, San Diego, CA) was resuspended in sterile water and stored at 4°C. Cells were treated after 24 hours from seeding in 6-well plates at the doses indicated in each figure. Drugs were diluted in full media and final DMSO concentration on cells never exceeded 0.01%.
Vincristine
Manufactured by Adipogen
Sourced in United States
Vincristine is a pure compound that is used as a reference standard or active pharmaceutical ingredient (API) in research and development. It is a cytotoxic alkaloid derived from the Vinca rosea plant.
Automatically generated - may contain errors
Lab products found in correlation
Doxorubicin, by Merck Group (2 mentions)
Gsk126, by Adooq (1 mentions)
Tetrandrine, by Merck Group (1 mentions)
Topotecan, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Ca 074me, by Selleck Chemicals (1 mentions)
5 fluorouracil, by Merck Group (1 mentions)
Naringenin, by Merck Group (1 mentions)
2 protocols using vincristine
1
Cell Line Drug Treatment Protocol
2
Comprehensive Compound Procurement Protocol
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Doxorubicin (10 mM in water), topotecan (10 mM in water), naringenin (100 mM in DMSO), chloroquine (100 mM in water), and 5-fluorouracil (50 mM in water) were purchased from Sigma Aldrich (Taufkirchen, Germany). Vincristine (100 mM in DMSO) was purchased from Adipogen (San Diego, CA, USA). Tetrandrine (10 mM in DMSO) was purchased from Sigma Aldrich and purified by the group of Prof. Franz Bracher (Ludwig-Maximilians University, Munich). CA-074Me (50 mM in DMSO) was purchased from Selleckchem (Houston, TX, USA).
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