Human recombinant transforming growth factor β tgf β

Manufactured by Thermo Fisher Scientific

Sourced in United States

Human recombinant transforming growth factor-β (TGF-β) is a cytokine that plays a key role in the regulation of cell growth, proliferation, and differentiation. It is a member of the transforming growth factor beta superfamily of proteins.

Automatically generated - may contain errors

Lab products found in correlation

Anti mouse cd3, by BD (4 mentions) Anti human cd4 microbeads, by Miltenyi Biotec (4 mentions) Anti mouse cd4 microbeads, by Miltenyi Biotec (4 mentions) Anti il 4, by Thermo Fisher Scientific (4 mentions) Soluble anti mous cd28, by BD (4 mentions) Anti ifn γ, by BD (4 mentions) Anti gapdh antibody, by Merck Group (1 mentions) Collagen 1, by Abcam (1 mentions) E cadherin, by Proteintech (1 mentions) Superoxide dismutase 2 sod2, by Proteintech (1 mentions) Anti alpha smooth muscle actin αsma antibody, by Merck Group (1 mentions) Sirtuin 3 sirt3, by Proteintech (1 mentions) 4 hydroxynonenal 4 hne, by Abcam (1 mentions)

Spelling variants of this product

TGF-β (625 citations) Recombinant human TGF-β (57 citations) Recombinant TGF-β (27 citations) Human TGF-β (25 citations) Transforming growth factor-β (TGF-β) (13 citations) Transforming growth factor-β (7 citations) TGFß (6 citations) Transforming growth factor (TGF)β (3 citations)

6 protocols using human recombinant transforming growth factor β tgf β

Isolation and Induction of Regulatory T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse splenic CD4 + T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4 + T-cells were cultured with plate-bound anti-mouse CD3 (1 μg/mL; BD Biosciences), soluble anti-mous CD28 (1 μg/mL; BD Biosciences), anti-IFN-γ (2 μg/ml), anti-IL-4 (2 μg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 μM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4 + T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4 + T-cells were cultured with plate-bound anti-human CD3 (1 μg/mL), soluble anti-human CD28 (1 μg/mL), anti-IFN-γ (2 μg/ml), anti-IL-4 (2 μg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 μM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~ 95% pure CD4 + CD25 + population.

Park M.J., Baek J.A., Kim S.Y., Jung K.A., Choi J.W., Park S.H., Kwok S, & Cho M.L. (2020). Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host disease with combination of regulatory T cells. Journal of Translational Medicine, 18, 483.

+ Open protocol

+ Expand

Isolation and Induction of Murine and Human Tregs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse splenic CD4+T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4+ T-cells were cultured with plate-bound anti-mouse CD3 (1 μg/mL; BD Biosciences), soluble anti-mous CD28 (1 μg/mL; BD Biosciences), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 μM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4+T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4+T-cells were cultured with plate-bound anti-human CD3 (1 μg/mL), soluble anti-human CD28 (1 μg/mL), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 μM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~95% pure CD4+CD25+ population.

Park M., Baek J., Kim S.Y., Jung K., Choi J.W., Park S., Kwok S., & Cho M. (2020). Myeloid-derived suppressor cells therapy enhance immunoregulatory properties in acute graft versus host diseas with combination of regulatory T cells.

+ Open protocol

+ Expand

Isolation and Expansion of Regulatory T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse splenic CD4 + T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4 + T-cells were cultured with plate-bound anti-mouse CD3 (1 µg/mL; BD Biosciences), soluble anti-mous CD28 (1 µg/mL; BD Biosciences), anti-IFN-γ (2 µg/ml), anti-IL-4 (2 µg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 µM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4 + T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4 + T-cells were cultured with plate-bound anti-human CD3 (1 µg/mL), soluble anti-human CD28 (1 µg/mL), anti-IFN-γ (2 µg/ml), anti-IL-4 (2 µg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 µM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~ 95% pure CD4 + CD25 + population.

Park M., Baek J., Kim S.Y., Jung K., Choi J.W., Park S., Kwok S., & Cho M. (2020). Myeloid-Derived Suppressor Cells Therapy Enhance Immunoregulatory Properties in Acute Graft Versus Host Diseas with Combination of Regulatory T Cells.

+ Open protocol

+ Expand

Isolation and Induction of Murine and Human Tregs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse splenic CD4+T cell were isolated from spleen by using mouse anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate Treg cells, CD4+ T-cells were cultured with plate-bound anti-mouse CD3 (1 μg/mL; BD Biosciences), soluble anti-mous CD28 (1 μg/mL; BD Biosciences), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant transforming growth factor-β (TGF-β; 5 ng/mL; PeproTech, London, UK), and Retinal (0.1 μM; Sigma-Aldrich, St. Louis, MO)(16).
Human CD4+T cells were isolated from PBMC by using human anti-CD4 microbeads (Miltenyi Biotec, Germany). To isolate human Treg cells, human CD4+T-cells were cultured with plate-bound anti-human CD3 (1 μg/mL), soluble anti-human CD28 (1 μg/mL), anti-IFN-g (2 mg/ml), anti-IL-4 (2 mg/ml), human recombinant TGF-β (5 ng/mL) and Retinal (0.1 μM).
After three days, the induced Treg cells were stained with CD4, CD25 and then sorted using a MoFlo cell sorter to obtain a ~95% pure CD4+CD25+ population.

+ Open protocol

+ Expand

TGF-β treatment of MCF10A cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human transforming growth factor β (TGF-β) was purchased from PeproTech (Rocky Hill, NJ, USA) and reconstituted according to the manufacturer's instructions. The isogenic MCF10A panel presented in Fig. 1A was cultured in vehicle control or 5 ng/ml TGF-β for 72 h before harvest.

Lourenco C., Kalkat M., Houlahan K.E., De Melo J., Longo J., Done S.J., Boutros P.C, & Penn L.Z. (2019). Modelling the MYC-driven normal-to-tumour switch in breast cancer. Disease Models & Mechanisms, 12(7), dmm038083.

+ Open protocol

+ Expand

Antifibrotic Effects of AKF-PD

Check if the same lab product or an alternative is used in the 5 most similar protocols

AKF-PD (lot No. 20190810) was purchased from Haikou Pharma (Haikou, China). ATP assays were obtained from Beyotime Biotechnology (Shanghai, China). Recombinant human transforming growth factor-β (TGF-β) was provided by PeproTech (Rocky Hill, USA). MitoSOX Deep Red was purchased from Invitrogen (New York, USA). The following antibodies for immunohistochemistry and western blotting were used: sirtuin 1 (SIRT1), NOX4, E-cadherin, superoxide dismutase 2 (SOD2), and sirtuin 3 (SIRT3) antibodies, which were purchased from Proteintech (San Diego, USA). Other antibodies were from Abcam (Cambridge, UK): collagen I, collagen III, fibronectin (FN), total OXPHOS complexes, and 4-hydroxynonenal (4HNE). Anti-alpha smooth muscle actin (α-SMA) antibody and anti-GAPDH antibody were obtained from Sigma (St. Louis, MO, USA) and Cell Signaling Technology (Boston, MA, USA), respectively. All other chemicals were of analytical grade.

Liao X., Lv X., Zhang Y., Han Y., Li J., Zeng J., Tang D., Meng J., Yuan X., Peng Z., Tao L, & Xie Y. (2022). Fluorofenidone Inhibits UUO/IRI-Induced Renal Fibrosis by Reducing Mitochondrial Damage. Oxidative Medicine and Cellular Longevity, 2022, 2453617.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!