Fumonitest

Aflatoxin B1 standard (1 mg, product number: A6636), aflatoxins mixture (B1, B2, G1, G2; product number: 34036), and the liquid mixture of fumonisins FB1 and FB2 (∼50 μg/mL in acetonitrile: water, product number: 34143) were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA) and fumonisin standard FB1 (1 mg, Item No. 62580) was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Working solutions were prepared according to the specifications of AOAC 991.31B.
HPLC grade reagents, methanol (99.8% purity), 10X phosphate buffer solution (PBS), 0.1% Tween-20/2.5% PEG/PBS 5X, AflaTest™ and FumoniTest™ immuno-affinity columns, Developer, and calibration standards for aflatoxins and fumonisins (AflaTest and FumoniTest) were purchased from VICAM (Milford, MA, EE.UU.).

AFM₁ standard was purchased from Sigma Aldrich (St. Louis, MO, USA) while FB₁ was acquired from PROMEC Unit of South Africa Medical Research Council (Tygerberg, Cape Town, South Africa). For toxins detection in urine, VICAM (Milford, MA, USA) AflaTest® WB and FumoniTest® WB immunoaffinity columns were used, while AflaTest® and FumoniTest® immunoaffinity columns were employed for food mycotoxin analyses. All other solvents were purchased from Fisher Scientific (Waltham, MA, USA) and were LC/MS grade. Ultrapure deionized water (18.2 MΩ cm) was used in all procedures that required water, except on methods that required LC/MS water.