Anti myc antibody sc 789

Polyclonal rabbit antibodies against Rpl35 (kindly provided by M. Seedorf, dilution 1:5000), Hem15 and Aco1 (kindly provided by R. Lill, dilution 1:7000 and 1:2000 respectively), Mex67 (kindly provided by C. Dargemont, dilution 1:50000), Rio2 (kindly provided by K. Karbstein, dilution 1:2000), Dbp5 (dilution 1:1000) and Rps3 (dilution 1:1000) were used. GFP-tagged proteins were detected with an anti-GFP antibody (sc-8334; Santa Cruz, dilution 1:1000) and myc-tagged proteins with an anti-myc antibody (sc-789; Santa Cruz, dilution 1:750). Monoclonal mouse antibodies against Pab1 (Santa Cruz, dilution 1:1000) and GST (sc-138; Santa Cruz, dilution 1:2000) and secondary anti-rabbit IgG (H+L)-HRPO and anti-mouse IgG (H+L)-HRPO (Dianova) antibodies were used. The signals were detected with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and the FUSION-SL chemiluminescence detection system (Peqlab) and the Western blot analyses were quantified using the Bio1D software (Peqlab).

Two‐week‐old ROX‐Myc^OsERF48 and NT plants grown on soil were hydroponically adapted in water for 3 days and then fixed by cross‐linking with 1% formaldehyde under vacuum for 15 min. Cross‐linking was stopped by the addition of glycine to a final concentration of 125 mm and application of vacuum for 10 min. After washing the plants in cold water, roots were collected and frozen in liquid nitrogen, and stored at −80 °C. The ChIP assay was performed as described by Chung et al. (2009), except that an anti‐myc antibody (SC‐789; Santa Cruz Biotech, Santa Cruz, CA) used. The ChIP product was analysed via quantitative PCR on a Mx3000P real‐time PCR system (Agilent Technologies). The enrichment values were normalized to the input sample. Values are the means ± SD of three independent experiments. All primer sequences are listed in Table S3.