Rabbit igg solution

The procedure for immobilization of bioreceptor and target is similar to the one described in section 4.4.1. Four diazonium modified SiCN chips (A2, B2, C2, D2) were incubated in 100 µg/mL goat anti-rabbit IgG solution (polyclonal, Sigma-Aldrich) for 2 h, and 5% BSA (‎bovine serum albumin) for 1 h at room temperature. Chips A2 and B2 were incubated in 200 µg/mL rabbit IgG solution (polyclonal, Sigma-Aldrich) for 1 h whereas chips C2 and D2 were subject to 200 µg/mL goat IgG solution (polyclonal, Sigma-Aldrich) as control for 1 h at room temperature. Chips A2 and C2 were immersed in 1:200 diluted FITC conjugated anti-rabbit IgG solution (Sigma-Aldrich) while Chips B2 and D2 were immersed in 1:400 diluted FITC conjugated anti-rabbit IgG solution at room temperature for 1 h.

Immobilization of recognition bioreceptor: Four SiCN chips (A1, B1, C1, D1) baring activated carboxyl groups were incubated in 100 µg/mL goat anti-rabbit IgG solution (polyclonal, Sigma-Aldrich) at room temperature for 2 h. The chips were rinsed with PBST (Phosphate Buffered Saline Tween-20) and then incubated in 5% BSA (‎bovine serum albumin) at room temperature for 1 h to block non-specific binding sites. The chips were then rinsed once again in PBST.
Immobilization of target: Two chips (A1 and B1) were immobilized with the detection target by incubating in 200 µg/mL rabbit IgG solution (polyclonal, Sigma-Aldrich) at room temperature for 1 h. As parallel control experimnent, the other two chips (C1 and D1) were incubated in 200 µg/mL goat IgG solution (polyclonal, Sigma-Aldrich) at room temperature for 1 h. The chips were then rinsed once again in PBST.
Immobilization of detection marker: Chips A1 and C1 were immersed in 40 nm AuNP (4.5 × 10¹¹ /mL) conjugated anti-rabbit IgG solution (10 µg/mL, Ted Pella) at room temperature for 1 h. Chips B1 and D1 were immersed in 1:3 diluted solution of 40 nm AuNP conjugated anti-rabbit IgG at room temperature for 1 h. Following incubation, the chips were water rinsed and dried under nitrogen flow. Table 3 summarizes the conditions under which each sample was prepared.