Crp 2 latex x2

Systolic and diastolic blood pressures were measured on the left upper arm after 5 minutes of rest in a sitting position using an automatic blood pressure monitor (HEM-907-E; OMROM, Tokyo, Japan). Abdominal circumference was measured in a standing position at the midway between the lower costal margin and the iliac crest. After fasting for 8 hours or more, blood was drawn from the antecubital vein of each participant. The samples were properly processed, refrigerated at 2℃ to 8℃, and analyzed within 24 hours of transportation. Fasting glucose was measured using a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan). High-performance liquid chromatography-723G7 (Tosoh, Tokyo, Japan) was used to check HbA1c. Insulin concentrations were estimated using an electrochemiluminescence method (COBAS E 411; Roche Diagnostics, Mannheim, Germany). The intra-assay and interassay coefficients of variation for the biochemical assays ranged between 3.1% and 7.6%. The blood levels of total cholesterol, triglycerides, high density lipoprotein, and low density lipoprotein cholesterol levels were measured using a Toshiba 200FR Autoanalyser (Toshiba Medical Systems Co. Ltd., Tokyo, Japan). High-sensitivity C-reactive protein was measured with an immunoturbidimetric method (CRP II Latex X2; Denka Seiken Co. Ltd., Tokyo, Japan) using an autoanalyzer (Toshiba).

Serum glucose, total cholesterol, triglycerides, high density–lipoprotein cholesterol, and low density–lipoprotein cholesterol were determined by enzymatic methods using commercially available kits according to instructions. Serum CRP was measured with an autoanalyzer (Toshiba, Tokyo, Japan) and immunoturbidimetric analysis (CRP II Latex X2; Denka Seiken, Tokyo, Japan). Serum-ferritin levels were evaluated using an ELISA kit (RCD012R, BioVendor). Estimation of serum LDH was performed using an LDH-activity assay kit, (7D69-20, Abbott Diagnostics). Plasma D-dimer was measured with an ELISA kit (NB 110–8376, Novus Biologicals). Lymphocyte count were performed with an autohematology analyzer (Sysmex BC 3000).