Excited-state decay kinetics were measured at room temperature, using a FluoTime 200 spectrometer (PicoQuant, Germany) equipped with a microchannel plate detector (Hamamatsu, Japan) and a PicoHarp 300 TCSPC set-up (PicoQuant) previously described in detail81 (link). In brief, a WhiteLase Micro supercontinuum laser (Fianium, UK) was used as a source of 540 nm excitation pulses. Fluorescence emission was detected through a monochromator in a wavelength range between 560 and 620 nm with 6 nm step size and binned in 4 ps time channels. Fluorescence decays were recorded at room temperature. Samples were diluted to OD 0.015 at the excitation wavelength in 1.5 mm path-length flow cell. The instrument response function (IRF) was measured at the excitation wavelength using 5% Ludox as scattering solution. The width of IRF was 40 ps. Global lifetime analysis of the fluorescence decays and iterative convolution with the measured IRF was performed using homebuilt MATLAB routines.
Fluotime 200 spectrometer
Manufactured by PicoQuant
Sourced in Germany
The FluoTime 200 is a compact, high-performance time-resolved fluorescence spectrometer designed for steady-state and time-resolved fluorescence measurements. It features a tunable pulsed light source, monochromators for excitation and emission, and a high-sensitivity detector. The FluoTime 200 enables the user to perform a wide range of fluorescence experiments and analyses.
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Lab products found in correlation
Microchannel plate detector, by Hamamatsu Photonics (1 mentions)
K alpha xps spectrometer, by Thermo Fisher Scientific (1 mentions)
Rf 5301pc spectrometer, by Shimadzu (1 mentions)
A300 spectrometer, by Bruker (1 mentions)
Xpert 3 x ray diffractometer, by Malvern Panalytical (1 mentions)
Tecnai f20, by Thermo Fisher Scientific (1 mentions)
Evo ma10, by Zeiss (1 mentions)
Uv 3600 spectrophotometer, by Shimadzu (1 mentions)
2 protocols using fluotime 200 spectrometer
1
Excited-State Fluorescence Decay Kinetics
2
Comprehensive Characterization of Nanomaterials
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The surface morphology was observed by Zeiss EVO MA10 scanning electron microscope (SEM) and FEI Tecnai F20 field-emission transmission electron microscope (TEM). The elemental mapping was collected by the FEI Tecnai F20 equipped with an energydispersive X-ray spectroscope (EDS) under STEM mode. X-ray diffraction (XRD) patterns were measured with PANalytical X'Pert3 X-ray diffractometer. X-ray photoelectron spectroscopy (XPS) was performed by the Thermo Scientific K-Alpha + XPS spectrometer. All binding energies were calibrated with respect to C 1 s peak at 284.8 eV. Electron paramagnetic resonance (EPR) spectra were measured with a Bruker A300 spectrometer. Raman spectra were collected by a HORIBA iHR320 Raman microscope with a 514-nm laser. UV-visible absorption spectra were measured using Shimadzu UV-3600 spectrophotometer with an integrated sphere. Photoluminescence (PL) spectra were analyzed using Shimadzu RF-5301PC spectrometer. Time-resolved photoluminescence (TRPL) spectra were measured by the PicoQuant Fluo Time 200 spectrometer coupled with a TimeHarp 260 time-correlated single-photon counting (TCSPC) system.
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