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Mouse anti ha antibody clone 16b12

Manufactured by BioLegend
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The Mouse anti-HA antibody (clone 16B12) is a monoclonal antibody that recognizes the hemagglutinin (HA) epitope tag. It is commonly used for the detection and purification of recombinant proteins expressing the HA tag.

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Lab products found in correlation

Passive lysis buffer (plb), by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti flag clone m2, by Merck Group (1 mentions) Irdye 680cw goat anti mouse igg, by LI COR (1 mentions) Odyssey clx infrared imaging system software, by LI COR (1 mentions)

Close spelling variants of this product

Anti-HA (86 citations) Mouse anti-HA (41 citations) Anti-HA antibody (37 citations) Anti-HA antibody (clone 16B12, (10 citations) Mouse anti-HA (clone 16B12; (8 citations) Anti-HA antibody (16B12, (6 citations) Mouse anti-HA (16B12, (5 citations) Anti-HA (16B12, (4 citations) Anti-HA (clone 16B12, (3 citations) Mouse anti-HA antibody (16B12) (2 citations)

2 protocols using mouse anti ha antibody clone 16b12

1

HIF-2α Transcriptional Activity Assay

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End-point Luciferase assays were performed as previously described. 20, 26 Real-time Luciferase assays were performed on HEK 293T cells transfected with jetPRIME ® (Ozyme Polyplus). Expression vectors pcDNA3-HA-PHD2 were co-transfected with pcDNA3-HA-HIF-2α, pGL3 3xHRE-luciferase reporter plasmid and pCMV-HA-empty vector. Luciferase activity was measured over 24 h using the bioluminometer WSL-1565 Kronos HT ® (ATTO). Cells were harvested at different timepoints and lysed in passive lysis buffer (Invitrogen) for immunoblot detection with a mouse anti-HA antibody (clone 16B12, BioLegend).
Delamare M., Le Roy A., Pacault M., Schmitt L., Garrec C., Maaziz N., Myllykoski M., Rimbert A., Karaghiannis V., Aral B., Catherwood M., Airaud F., Mansour-Hendili L., Hoogewijs D., Peroni E., Idriss S., Lesieur V., Caillaud A., Si-Tayeb K., Chariau C., Gaignerie A., Rab M., Haferlach T., Meggendorfer M., Bézieau S., Benetti A., Casadevall N., Hirsch P., Rose C., Wemeau M., Galacteros F., Cassinat B., Bellosillo B., Bento C., Van Wijk R., Petrides P.E., Randi M.L., McMullin M.F., Koivunen P., Girodon F, & Gardie B. (2023). Characterization of genetic variants in the EGLN1/PHD2 gene identified in a European collection of patients with erythrocytosis. Haematologica, 108(11).
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2

Western Blotting for Plasmodium Parasites

Cited 2 times
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Western blotting for Plasmodium parasites was performed as described previously (35 (link), 64 (link)). Briefly, parasites were selectively permeabilized by treatment with ice-cold 0.03% saponin in PBS for 15 min followed by lysis with RIPA to remove the hemozoin. The antibodies used in this study were mouse anti-HA antibody (clone 16B12, BioLegend; 1:500), rabbit polyclonal anti-HA SG77 (ThermoFisher; 1:500), mouse monoclonal anti-FLAG clone M2 (Sigma-Aldrich; 1:200), and mouse monoclonal anti-EXP2 clone 7.7 (1:500). The secondary antibodies used are IRDye 680CW goat anti-mouse IgG (LICOR Biosciences) (1:20,000). Western blot images were processed using the Odyssey Clx LICOR infrared imaging system software (LICOR Biosciences). Full-length blots are presented in Fig. S5.
Fierro M.A., Muheljic A., Sha J., Wohlschlegel J, & Beck J.R. (2024). PEXEL is a proteolytic maturation site for both exported and non-exported Plasmodium proteins. mSphere, 9(2), e00393-23.
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