All reagents were endotoxin free. rhGM-CSF and rhTNF-α were from PeproTech (Rocky Hill, NJ, USA); IL-4 and IFN-γ were from R&D Systems Europe (Oxford, United Kingdom); Ultrapure TLR4 agonist (Salmonella Minnesota LPS) was from InvivoGen (San Diego, CA, USA); rhIL-1β and rhIL-6 were from ImmunoTools (Friesoythe, Germany); IL-23 was from eBioscience (San Diego, CA, USA); and PGE2, indomethacin, and forskolin were from Sigma-Aldrich (Dorset, United Kingdom). Mouse anti-human CD4-PE was from BD Biosciences (Oxford, United Kingdom); Mouse anti-human CD4-PE-Cy7, mouse anti-human CD45RA-FITC, mouse anti-human CD14-PE-Cy5.5, and matching isotype controls were from eBioscience. For CD4 activation, mouse anti-human CD28 was obtained from BD Biosciences, IL-2 from R&D Systems Europe, and CD3 (OKT3) was produced in-house. The annexin V/PI staining kit was obtained from BD Biosciences.
Mouse anti human cd4 pe cy7
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The Mouse anti-Human CD4-PE-Cy7 is a flow cytometry reagent used for the detection and quantification of CD4+ T cells in human samples. It contains a mouse monoclonal antibody that binds specifically to the CD4 surface antigen on human T cells. The antibody is conjugated to the fluorescent dyes Phycoerythrin (PE) and Cyanine 7 (Cy7), which allow for the identification and enumeration of CD4-positive cells by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti human cd4 pe, by BD (2 mentions)
Indomethacin, by Merck Group (2 mentions)
Il 23, by Thermo Fisher Scientific (2 mentions)
Forskolin, by Merck Group (2 mentions)
Mouse anti human cd28, by BD (2 mentions)
Ultrapure tlr4 agonist salmonella minnesota lps, by InvivoGen (2 mentions)
Mouse anti human cd45ra fitc, by Thermo Fisher Scientific (2 mentions)
Mouse anti human cd14 pe cy5, by Thermo Fisher Scientific (2 mentions)
Interleukin 4 (il 4), by R&D Systems (2 mentions)
Ifn γ, by R&D Systems (2 mentions)
Annexin 5 pi staining kit, by BD (2 mentions)
Rhtnf α, by Thermo Fisher Scientific (1 mentions)
Rhgm csf, by Thermo Fisher Scientific (1 mentions)
Interleukin 2 (il 2), by R&D Systems (1 mentions)
Rh il 6, by Immunotools (1 mentions)
Recombinant human gm csf, by Thermo Fisher Scientific (1 mentions)
Interleukin 2 (il 2), by BD (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Recombinant human il 1β, by Immunotools (1 mentions)
2 protocols using mouse anti human cd4 pe cy7
1
Cytokine-Induced Monocyte Activation
2
Human Dendritic Cell Differentiation
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All reagents were endotoxin-free. Recombinant human GM-CSF and TNFα were from PeproTech, Rocky Hill, NJ; IL-4 and IFNγ were from R&D Systems Europe, Oxford, U.K.; Ultrapure TLR4-agonist (Salmonella Minnesota LPS) was from InvivoGen (San Diego, CA); Recombinant human IL-1β and IL-6 were from Immunotools, Friesoythe, Germany; IL-23 was from eBioscience (San Diego, CA); PGE 2 , Indomethacin and forskolin were from Sigma-Aldrich (Dorset, U.K.). Mouse anti-human CD4-PE was from BD Biosciences (Oxford, U.K.); Mouse anti-human CD4-PECy7, mouse anti-human CD45RA-FITC, mouse anti-human CD14-Pe-Cy5.5 and matching isotype controls were from eBioscience (San Diego, CA). For CD4 activation, mouse anti-human CD28 was obtained from BD Biosciences, IL-2 (R&D systems), and CD3 (OKT3) was produced in-house. AnnexinV/PI staining kit was obtained from BD Bioscience (Oxford, UK).
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