L ascorbic acid vc

NH300094 hydrochloride, risperidone and duloxetine hydrochloride were synthesized at Jiangsu Nhwa Pharmaceutical Co., Ltd. The molecular structure of NH300094 (PCT/CN 2020/129850) is shown in Figure 1. Other compounds such as (+)-MK-801 hydrogen maleate (M107-250MG, Sigma), rivastigmine hydrogen tartrate (LRAB1259, Sigma), R (2)-2,5-dimethoxy-4-iodoamphetamine (DOI) (D101-100MG, Sigma), R-(−)-Apomorphine (APO) hydrochloride (A4393, Sigma), L-Ascorbic acid Vc (A5960-25G, Sigma), (+)-Butaclamol (D033, Sigma) were purchased from Sigma-Aldrich (St. Louis, MO). Scopolamine hydrobromide (S107418-5g, Aladdin) was purchased from Aladdin. WAY-00635 (T2631, Targetmol), Ketanserin (T1066, Targetmol), Yohimbine (T2142, Targetmol), Pyrilamine (T1232, Targetmol), Atropine (T0375, Targetmol), Prazosin (T1050, Targetmol) were purchased from Targetmol. Methiothepin (HY-107836, MCE) was purchased from MCE. All drugs were dissolved in normal saline or deionized water and administered orally at 10 mL/kg (volume/body weight), unless otherwise indicated.

Silver nitrate (AgNO₃), polyvinylpyrrolidone (PVP), ethylene glycol, glycerol, sodium selenite (Na₂SeO₃), chitosan (Cs, molecular weight 150 kDa; deacetylation degree 85%), sodium chloride, l-ascorbic acid (Vc), 1-ethyl-3-[3-(dimethylamino)-propyl] carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich. RGD (H-Gly-Arg-Gly-Asp-Asn-Pro-OH) were purchased from Sangon Biotech (Shanghai) Co., Ltd. Fluorescein isothiocyanate (FITC), 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA), live/dead cell viability kit, BCA protein detection kit, mitochondrial membrane potential detection kit (JC-1), apoptosis detection kit (annexin V-FITC and propidium iodide) were purchased from ThermoFisher Scientific (China) Co., Ltd. All antibodies were obtained from Cell Signal Technology (United States).