The following reagents were from BD Biosciences (San Jose, CA, USA) or eBiosciences (San Diego, CA, USA): Monoclonal antibodies to CD11b-FITC (M1/70), F4/80-PerCP5.5, CD11c-APC, CD3-pacific blue, CD4-FITC, CD8-APC, CXCL9-PE, CXCR3/CD183-PE and their corresponding isotype antibodies (Rat IgG2a-FITC, Rat IgG2a-PerCP5.5, Armenian hamster IgG-APC, Rat IgG2a-pacific blue, Rat IgG2a-FITC, Rat IgG2a APC, Hamster IgG-PE). Recombinant murine CXCL9 from Pepro Tech (Cranbury, NJ, USA) and purified mouse anti-CXCR3 antibodies (catalog number-155902, clone-S18001A, and lot number-B265189) was from Biolegend (San Diego, CA, USA). Liberace Cl was from Roche (Indianapolis, IN, USA). Bovine serum albumin (BSA), Gey’s balanced salt solution (GBSS), and DNase were from Sigma (St. Louis, MO, USA). Anti-CD11b, CD11c, CD4, and CD8 microbeads were from Miltenyi Biotec (Auburn, CA, USA). Diff-Quik stain set was from Dade Behring, Inc. (Newark, NJ, USA). Polycarbonate membranes, cell scraper, and Boyden chemotaxis chamber were from Neuro Probe, Inc. (Gaithersburg, MD, USA). ELISA kit for the detection of mouse CXCL9 was from R&D System (Minneapolis, MN, USA). LSRII flow cytometer from BD Biosciences (San Jose, CA, USA), FCS Express software from De Novo Software (Los Angeles, CA, USA).
Fitc rat igg2a
Manufactured by Thermo Fisher Scientific
Sourced in United States
FITC rat IgG2a is a fluorescently labeled antibody. It is used as a tool in research applications to detect and quantify target proteins or cells.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β actin, by GeneTex (2 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Cd4 fitc, by Thermo Fisher Scientific (1 mentions)
Cd8 apc, by Thermo Fisher Scientific (1 mentions)
Dnase, by Merck Group (1 mentions)
Polycarbonate membrane, by Neuro Probe (1 mentions)
Fcs express software, by De Novo Software (1 mentions)
Recombinant murine cxcl9, by Thermo Fisher Scientific (1 mentions)
Armenian hamster igg apc, by Thermo Fisher Scientific (1 mentions)
Cd3 pacific blue, by Thermo Fisher Scientific (1 mentions)
Cd11c apc, by Thermo Fisher Scientific (1 mentions)
Cd11b fitc m1 70, by Thermo Fisher Scientific (1 mentions)
Boyden chemotaxis chamber, by Neuro Probe (1 mentions)
Rabbit anti stat3, by Cell Signaling Technology (1 mentions)
Mouse anti ass1, by BD (1 mentions)
Rabbit anti ccnd1, by Cell Signaling Technology (1 mentions)
Peroxidase conjugated goat anti rabbit igg, by Cell Signaling Technology (1 mentions)
Rabbit anti akt1, by Cell Signaling Technology (1 mentions)
Mouse anti cdh1, by BD (1 mentions)
3 protocols using fitc rat igg2a
1
Murine CXCR3 Chemokine Receptor Assay
2
Antibody Characterization in MNU-Treated Cells
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N-nitro-N-methylurea (MNU) was purchased from Sigma-Aldrich (St. Louis, MO). The following antibodies (Abs) were used in this study and were purchased from BD PharMingen (San Diego, CA): mouse anti-CD4 PE (H129.19), anti-CD8a PE (53-6.7); anti-CD11b PE (M1/70), anti-F4/80 PE (BM8), anti-Ly6G FITC (1A8), anti-Ly6C FITC (AL-21) mAb. The anti-CD44 PE (IM7), PE rat IgG1 and FITC rat IgG2a isotype control Abs were purchased from eBioscience. The following antibodies were used in this study: mouse anti-ASS1 (BD Transduction Laboratories, San Jose, CA, USA); anti-MST3 (EP1468Y) (Abcam, United Kingdom); mouse anti-JAK1 (BD Biosciences, San Jose, CA); rabbit anti-STAT3, rabbit anti-CCND1, rabbit anti-AKT1 and peroxidase-conjugated goat anti-rabbit IgG (Cell Signaling, Boston, MA, USA); mouse anti-β-actin (GeneTex, Inc., San Antonio, TX, USA); and peroxidase-conjugated sheep anti-mouse IgG (Chemica, San Diego, CA, USA).
3
Antibody-Based Immunophenotyping Protocol
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The following antibodies used in this study were purchased from BD PharMingen (San Diego, CA): mouse anti-CD4 PE (H129. 19 ), anti-CD8a PE (53-6.7), anti-CD11b PE (M1/70), anti-F4/80 PE (BM8), and anti-Ly6C FITC (AL-21) monoclonal antibodies. In addition, PE-conjugated anti-mouse CD44, PE rat IgG1, and FITC rat IgG2a isotype control antibodies were purchased from eBioscience (San Diego, CA). The remaining antibodies used were as follows: anti-MST3/STK24 (EP1468Y; Abcam, UK), mouse anti-CDH1 (BD Transduction Laboratories, San Jose, CA), rabbit anti-beta catenin (GeneTex, Inc., San Antonio, TX), rabbit anti-AKT1 and peroxidase-conjugated goat anti-rabbit IgG (Cell Signaling, Boston, MA), mouse antiβ-actin (GeneTex, Inc.), and peroxidase-conjugated sheep anti-mouse IgG (Chemica, San Diego, CA).
Cell culture MKN45 cells were kindly provided by Professor Ming-Derg Lai (National Cheng Kung University, Tainan, Taiwan). The MKN45 cell lines were authenticated in 2013 by DNA short tandem repeat pro ling at Bioresource Collection and Research Center. These cell lines were maintained in RPMI 1640 medium containing 10% fetal bovine serum (FBS) (Gibco, Life Technologies, Grand Island, NY) and 1% penicillin/streptomycin. M12 cells were maintained in Dulbecco's modi ed Eagle's medium/high glucose supplemented with 10% FBS and 1% penicillin/streptomycin.
Cell culture MKN45 cells were kindly provided by Professor Ming-Derg Lai (National Cheng Kung University, Tainan, Taiwan). The MKN45 cell lines were authenticated in 2013 by DNA short tandem repeat pro ling at Bioresource Collection and Research Center. These cell lines were maintained in RPMI 1640 medium containing 10% fetal bovine serum (FBS) (Gibco, Life Technologies, Grand Island, NY) and 1% penicillin/streptomycin. M12 cells were maintained in Dulbecco's modi ed Eagle's medium/high glucose supplemented with 10% FBS and 1% penicillin/streptomycin.
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