Tissues of all groups were fixed in phosphate-buffer containing 2.5% glutaraldehyde (Sigma-Aldrich Co.) for 2-3 h, post-fixed in 1% osmium tetraoxide (Sigma-Aldrich Co.) and dehydrated in a series of graded alcohols (50, 60, 70, 80, 90, 96 and 100% ethanol). After passing through propylene oxide (Sigma-Aldrich Co.), the specimens were embedded in Araldite CY 212 (Ciba-Geigy), (2-dodecen-1-yl)succinic anhydride (Sigma-Aldrich Co.), benzyldimethyl amine (Poly Sciences Inc.) and dibutylphtalate (Sigma-Aldrich Co.). The semi-thin sections were stained with toluidine blue (Sigma-Aldrich Co.) and examined with a photomicroscope (BH2 Olympus, Japan). After the selection of appropriate specimens, thin sections were cut and stained with uranyl acetate (ProSciTech) and lead citrate (Sigma-Aldrich Co.). They were examined by an electron microscope (Carl Zeiss EM 900, Germany).
Benzyldimethyl amine
Manufactured by Polysciences
Benzyldimethyl amine is a colorless liquid organic compound. It is used as a chemical intermediate in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Propylene oxide, by Merck Group (3 mentions)
Dibutylphtalate, by Merck Group (3 mentions)
Uranyl acetate, by ProSciTech (3 mentions)
Lead citrate, by Merck Group (3 mentions)
Toluidine blue, by Merck Group (3 mentions)
2 dodecen 1 yl succinic anhydride, by Merck Group (3 mentions)
Glutaraldehyde, by Merck Group (3 mentions)
Osmium tetraoxide, by Merck Group (3 mentions)
Em 900, by Zeiss (2 mentions)
Methylnadic anhydride, by Serva Electrophoresis (1 mentions)
Glycidether, by Serva Electrophoresis (1 mentions)
Dm4000, by Leica (1 mentions)
Dm500, by Leica (1 mentions)
Leo 906e, by Zeiss (1 mentions)
4 protocols using benzyldimethyl amine
1
Transmission Electron Microscopy Protocol for Tissue Analysis
2
Ultrastructural Cell Fixation and Embedding
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Cells were fixed with half-strength Karnovsky fixative (2% PFA, 2.5% GA, 0.1 M PB, pH 7.4) for 2 h at RT. After scraping, cells were pelleted and embedded in 2% low melting point agarose. The cell pellets were postfixed as previously described [80 (link)] with 1% OsO4, 1.5% K3Fe(CN)6, in 0.065 M Na-cacodylate, stained en bloc with 0.5% uranyl acetate, dehydrated in acetone and embedded in Epon composed of glycid ether (Serva, 21045.02), 2-dodecenylsuccinic acid anhydride (Serva, 20755.02), methyl nadic anhydride (Serva, 29452.03) and benzyldimethylamine (PolyScience, 00141–100) mixed in weight ratio 48:32:20:3. Ultrathin sections were stained with uranyl acetate and lead citrate.
3
Electron Microscopy Tissue Preparation
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Tissues of all groups were fi xed in phosphate buffer containing 2.5 % glutaraldehyde (Sigma-Aldrich Co.) for 2-3 h, post-fi xed in 1 % osmium tetra oxide (Sigma-Aldrich Co.) and dehydrated in a series of graded alcohols (50, 60, 70, 80, 90, 96 and 100 % ethanol). After passing through propylene oxide (Sigma-Aldrich Co.), the specimens were embedded in Araldite CY 212 (Ciba-Geigy), (2-dodecen-1-yl) succinic anhydride (Sigma-Aldrich Co.), benzyldimethyl amine (Poly Sciences Inc.) and dibutylphtalate (Sigma-Aldrich Co.). The semi-thin sections were stained with toluidine blue (Sigma-Aldrich Co.), and examined with a photomicroscope (Leica DM4000, Germany). After the selection of appropriate specimens, thin sections were cut and stained with uranyl acetate (Pro Sci Tech) and lead citrate (Sigma-Aldrich Co.). They were examined by means of an electron microscope (Carl Zeiss EM 900, Germany) (17) .
4
Electron Microscopy Tissue Preparation
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Tissues from all the groups were fixed in phosphate buffered solution (Ph= 7.3) containing 2.5% glutaraldehyde (Sigma-Aldrich Co.) for 2 h at room temperature, post-fixed in 1% osmium tetraoxide (Sigma-Aldrich Co.) and dehydrated in a series of graded alcohols (50, 60, 70, 80, 90 and 100% ethanol). After passing through propylene oxide (Sigma-Aldrich Co.), the specimens were embedded in Araldite CY 212 (Ciba-Geigy), (2-dodecen-1-yl) succinic anhydride (Sigma-Aldrich Co.), benzyldimethylamine (Poly Sciences Inc.) and dibutylphtalate (Sigma-Aldrich Co.). The semi-thin sections were stained with toluidine blue (Sigma-Aldrich Co.) and examined with a photomicroscope (DM 500 Leica, Germany). After the selection of appropriate specimens, thin sections were cut, stained with uranyl acetate (ProSciTech) and lead citrate (Sigma-Aldrich Co.), and examined via an electron microscope by two blinded histologists (Leo 906 e Carl Zeiss, Germany).
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