Plenti p2a mcherry vector

Manufactured by Takara Bio

The PLenti-P2A-mCherry vector is a lentiviral expression vector that allows for the expression of the mCherry fluorescent protein. The mCherry gene is separated from a gene of interest by a self-cleaving P2A peptide sequence, enabling the simultaneous expression of both the gene of interest and the mCherry reporter.

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Lab products found in correlation

Plvx tre3g vector, by Takara Bio (2 mentions)

2 protocols using plenti p2a mcherry vector

Cloning and Expression of AncBE4max

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The sequence of AncBE4max was obtained from supplementary information of Koblan et al. 28 and synthesized by Synbio Technologies. The AncBE4max construct was cloned into pLenti-P2A-mCherry vector and pLVX-TRE3G vector (TaKaRa, 631187) through double restriction enzyme digestion (NEB) and T4 ligase ligation (NEB, M0202). Individual sgRNA oligos were synthesized by Ruibotech and cloned into pCG-2.0 sgRNA-expressing vector through Golden-Gate assembly.

Xu P., Liu Z., Liu Y., Ma H., Xu Y., Bao Y., Zhu S., Cao Z., Wu Z., Zhou Z, & Wei W. (2021). Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs. Nature biotechnology, 39(11).

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Cloning and Expression of AncBE4max

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Xu P., Liu Z., Liu Y., Ma H., Xu Y., Bao Y., Zhu S., Cao Z., Zhou Z., & Wei W. (2020). Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs.

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