The formation of MDA was used as an indicator of lipid peroxidation via the thiobarbituric acid assay (MDA detection kit A003, Jiancheng Bioengineering Institute, Nanjing). Glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) activity were determined using kits from Nanjing Jiancheng Bioengineering Institute (CAT detection kit A0071-1, SOD detection kit A001-3, GSH-Px detection kit A005). The protein concentrations of the samples were measured using the Bradford method (Bradford, 1976 (link)).
Sod detection kit a001 3
Manufactured by Nanjing Jiancheng
Sourced in China
The SOD detection kit A001-3 is a laboratory product designed to detect the presence and quantify the level of superoxide dismutase (SOD) in various samples. The kit provides the necessary reagents and protocols to perform this analysis.
Automatically generated - may contain errors
2 protocols using sod detection kit a001 3
1
Lipid Peroxidation and Antioxidant Enzyme Assays
2
Hepatic Antioxidant and Lipid Measurement
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Hepatic TG levels were determined using kits (TG detection kit A110-2-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Hepatic glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), and catalase (CAT) activity were also determined using kits (GSH-Px detection kit A005, SOD detection kit A001-3, and CAT detection kit A0071-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Hepatic malondialdehyde (MDA) levels were determined using a thiobarbituric acid assay (MDA detection kit A003-1; Jiancheng Bioengineering Institute, Nanjing, China). All indices were measured according to the manufacturer's instructions. The protein concentrations of the samples were measured using the Bradford method (34 (link)).
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