Mouse spleens were embedded in Cryomatrix™ embedding resin (Thermo Fisher Scientific). Frozen tissues were sectioned at 7 μm thick using CryoStar™ NX70 Cryostat (Thermo Fisher Scientific), then fixed in acetone, air-dried, and rinsed in PBS. Next, spleen cryosections were stained with anti-B220-biotin (BD Biosciences), GL7-Alexa Fluor 488 (eBioscience), and Ki-67-Horizon V450 (BD Biosciences), followed by incubation with Alexa Fluor 594-conjugated streptavidin (Invitrogen). Slides were mounted with ProLong™ gold antifade mounting media (Thermo Fisher Scientific). Confocal images were acquired using a TCS SPE confocal microscope (Leica) with a 60× zoom. The data were analyzed using LAS X Leica software (Leica).
Gl7 alexa fluor 488
Manufactured by Thermo Fisher Scientific
The GL7-Alexa Fluor 488 is a fluorescent dye that can be used for various experimental applications. It is a conjugate of the dye Alexa Fluor 488 and the GL7 antibody, which is specific for germinal center B cells. The Alexa Fluor 488 dye emits green fluorescence when excited by a suitable light source, allowing for the detection and analysis of GL7-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Leica las x software, by Leica camera (2 mentions)
Ki 67 horizon v450, by BD (2 mentions)
Tcs spe confocal microscope, by Leica camera (2 mentions)
Cryomatrix embedding resin, by Thermo Fisher Scientific (2 mentions)
Anti b220 biotin, by BD (2 mentions)
Alexa fluor 594 conjugated streptavidin, by Thermo Fisher Scientific (2 mentions)
Cryostar nx70 cryostat, by Thermo Fisher Scientific (2 mentions)
Prolong gold antifade mounting media, by Thermo Fisher Scientific (2 mentions)
2 protocols using gl7 alexa fluor 488
1
Multicolor Immunofluorescence Staining of Mouse Spleen
2
Immunofluorescent Staining of Mouse Spleen
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Mouse spleens were embedded in Cryomatrix™ embedding resin (Thermo Fisher Scientific). Frozen tissues were sectioned at 7 µm thick using CryoStar™ NX70 Cryostat (Thermo Fisher Scientific), then fixed in acetone, air-dried, and rinsed in PBS. Next, spleen cryosections were stained with anti-B220-biotin (BD Biosciences), GL7-Alexa Fluor 488 (eBioscience), and Ki-67-Horizon V450 (BD Biosciences), followed by incubation with Alexa Fluor 594-conjugated streptavidin (Invitrogen). Slides were mounted with ProLong™ gold antifade mounting media (Thermo Fisher Scientific).
Confocal images were acquired using a TCS SPE confocal microscope (Leica) with a 60 × zoom. The data were analyzed using LAS X Leica software (Leica).
Confocal images were acquired using a TCS SPE confocal microscope (Leica) with a 60 × zoom. The data were analyzed using LAS X Leica software (Leica).
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