PLGA (75:25 L:G; ester-terminated, inherent viscosity range: 0.55–0.75 dL/g in CHCl3) was purchased from Lactel. All lipids for the nanoparticle synthesize were purchased from Avanti Polar Lipids, including 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG-MAL). Cholesteryl butyrate was purchased from Santa Cruz Biotechnology. Rhesus recombinant anti-CD4 antibody and rhesus recombinant IgG1 isotype control antibody were purchased from NIH Nonhuman Primate Reagent Resource. FITC mouse anti-human CD8, PE mouse anti-human CD14, PerCP mouse anti-human CD3 antibodies, FITC mouse anti-human CD4, and FITC mouse IgG1, PE mouse IgG2a, PerCP mouse IgG1 κ control antibodies were purchased from BD Biosciences. RPMI 1640 containing 2 mMl -glutamine and 25 mM HEPES, DPBS, heat-inactivated fetal bovine serum (FBS), Penicillin–Streptomycin (10,000 U/mL), 1,1′ -Dioctadecyl-3,3,3′, 3′ -Tetramethylin-dodicarbocyanine, 4-Chlorobenzenesulfonate Salt (DiD), LIVE/DEAD® Fixable Violet Dead Cell Stain Kit, Dylight 633 NHS Ester were purchased from ThermoFisher. All other chemicals were purchased from Sigma-Aldrich and Fisher Scientific unless otherwise specified.
Dspe peg mal
Manufactured by Avanti Polar Lipids
Sourced in United States
DSPE-PEG-Mal is a lipid-based compound that contains a maleimide functional group. It is commonly used in the development and preparation of liposomes and other lipid-based drug delivery systems.
Automatically generated - may contain errors
Lab products found in correlation
Fitc mouse anti human cd4, by BD (1 mentions)
Mouse igg1 fitc, by BD (1 mentions)
Pe mouse anti human cd14, by BD (1 mentions)
Fitc mouse anti human cd8, by BD (1 mentions)
Mouse igg2a pe, by BD (1 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Stempro accutase, by Thermo Fisher Scientific (1 mentions)
Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated antibody, by R&D Systems (1 mentions)
6 well ultra low attachment surface dishes, by Corning (1 mentions)
4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (1 mentions)
Pe cf, by Avanti Polar Lipids (1 mentions)
Cd133 microbead kit, by Miltenyi Biotec (1 mentions)
1 2 ethanedithiol edt, by Merck Group (1 mentions)
1 4 dithiothreitol dtt, by Merck Group (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (1 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n maleimide polyethylene glycol 2000, by Avanti Polar Lipids (1 mentions)
Nanoassemblr, by Precision Nanosystems (1 mentions)
Cholesterol, by Avanti Polar Lipids (1 mentions)
4 protocols using dspe peg mal
1
Nanoparticle Synthesis and Characterization
2
Aptamer-conjugated PLGA Nanoparticles for CD133+ Cell Targeting
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Poly(DL-lactide-co-glycolide) (PLGA) with a molar ratio of lactide:glycolide of 50:50 (40–75 kDa), polyvinyl alcohol (PVA; 30–70 kDa), RA and organic reagents were all purchased from Sigma-Aldrich; EMD Millipore (Billerica, MA, USA). The lipids, including [(1,2-distearoyl-sn-glycero-3- phosphoethanolamine-N-maleimide- polyethylene glycol-2000; DSPE-PEG-Mal, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-carboxyfluorescein; PECF)], phosphatidylcholine, and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The CD133 MicroBead kit was provided by Miltenyi Biotec, Inc. (Shanghai, China). The ultra-low attachment surface 6-well dishes were purchased from Corning Life Sciences (Tewksbury, MA, USA). R&D Systems, Inc. (Minneapolis, MN, USA) provided recombinant anti-human CD133 Alexa Fluor® 488-conjugated antibody (cat. no. FAB11331G). The synthesis of CD133 aptamers with the sequence of 5′-SH-CCCUCCUACAUAGGG-3′ was performed by Ruibo Co., Ltd. (Guangzhou, China). The Pierce BCA Protein Assay kit, FBS, B27, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), insulin-transferrin-selenium (ITS), 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI) and the cell dissociation reagent (StemPro® Accutase®) were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA).
3
PEGylated Liposomal Doxorubicin Synthesis
4
Synthesis of Lipid Nanoparticles for RNA Delivery
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DLin‐MC3‐DMA (MC3), SM102, Lipid 10, and Lipid 14 were synthesized according to previously described method.[17 , 39 ] Cholesterol, DSPC (1,2‐ distearoyl‐sn‐glycero‐3‐phosphocholine), polyethylene glycol (PEG)‐DMG (1,2‐dimyristoyl‐rac‐glycerol), and DSPE‐PEG‐mal (1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐[maleimide(polyethylene glycol)‐200](ammonium salt) were purchased from Avanti Polar Lipids Inc. Briefly, one volume of lipid mixture (ionizable cationic lipid, Cholesterol, DSPC and PEG‐DMG at 50:38.5:10:1.5 molar ratio) in absolute ethanol and three volumes of siRNA (1:6 mole N to P ratio RNA to ionizable lipid) in a 25 × 10‐3m acetate buffer were injected into a NanoAssemblr microfluidic mixing device (Precision Nanosystems Inc.) at a combined flow rate of 12 mL min−1. For the preparation of targeted LNPs (tLNPs), lipid 10 or DLin‐MC3‐DMA, Cholesterol, DSPC, PEG‐DMG and DSPE‐PEG‐mal were mixed in the following ratio: 50:38:10:1.9:0.1. For preparation of LNPs‐Cy5‐LNPs, Cy5‐siRNA was used at 50% of total RNA amount. After LNPs generation, the particles were dialyzed against PBS (pH 7.4) for 24 h with two buffer exchanges to remove ethanol.
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