Olaparib o 9201

FBS (F0926), thiazolyl blue tetrazolium bromide (MTT) (M2128), cisplatin (232120), PVDF membranes (IPVH00010), and actin antibody (A1978) were purchased from Sigma-Aldrich (St. Louis. MO, USA). Trypsin-EDTA (25300–054), DMEM (11995-065), bovine serum albumin (BP1605-100), Col17A1 (MA5-24848), goat anti-mouse Alexa fluor 680 IgG (A21058), goat anti-rabbit Alexa fluor 680 IgG (A21109), and Lipofectamine 2000 (11-668-019) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Penicillin-streptomycin (SV30010) was purchased from GE Healthcare (Chicago, IL, USA). The PARP inhibitor olaparib (O-9201) was obtained from LC Laboratories (Woburn, MA, USA). The mTOR inhibitor rapamycin (13346), everolimus (11597), and chloroquine (30708) were obtained from Cayman Chemicals (Ann Arbor, MI, USA). The protein assay dye (500-0006) was purchased from Bio-Rad (Hercules, CA, USA). RIPA Buffer (9806), S100A9 (72590), p62 (5114), GAPDH (5174), and LC3 (2775) antibodies were purchased from Cell Signaling Technologies (Danvers, MA, USA).

YK-4-279 (S7679), Nutlin-3 (S1061), talazoparib (S7048), necrostatin (S8037), Z-VAD-FMK (S7023), and dabrafenib (S2807) were purchased from Selleck Chem (Houston, TX, USA), olaparib (O-9201) from LC Laboratories (Woburn, MA, USA), cisplatin and a 30% H₂O₂ solution was purchased from Sigma Aldrich (St Louis, MO, USA). All compounds, except cisplatin and H₂O_2, were dissolved in dimethylsulfoxid (DMSO; Sigma Aldrich) obtaining a final stock concentration of 10 mM. For cisplatin a 5 mM stock in cell culture medium was made. These compounds were used in a range from 0–50 µM.