2 7 dichlorofluorescindiacetate dcfh da

Manufactured by Yeasen

Sourced in China

2,7-Dichlorofluorescindiacetate (DCFH-DA) is a chemical compound commonly used as a fluorescent probe in cell-based assays. It is a cell-permeable, non-fluorescent molecule that can be oxidized to the highly fluorescent 2,7-dichlorofluorescein (DCF) upon reaction with reactive oxygen species (ROS) within cells. The fluorescence intensity of DCF is proportional to the level of ROS, making DCFH-DA a useful tool for detecting and quantifying intracellular oxidative stress.

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Lab products found in correlation

2 protocols using 2 7 dichlorofluorescindiacetate dcfh da

Hyaluronic Acid-based Nanoparticles for Doxorubicin Delivery

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Hyaluronic acid, propylene sulfide, adipic
dihydrazide, caprolactone, N-Boc-ethanolamine, doxorubicin
hydrochloride (DOX·HCl), dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine
(DMAP), ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride
(EDC·HCl), and N-hydroxysuccinimide (NHS) were
purchased from Sigma-Aldrich Co., Ltd. (Beijing, China). N,N-Dimethylformamide (DMF) and triethylamine (TEA)
were purchased from Alfa Aesar Co., Ltd. (Shanghai, China). 2,7-Dichlorofluorescin
diacetate (DCFH-DA) was purchased from Yeasen Biotechnology Co., Ltd.
(Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI), propidium
iodide (PI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT), and TUNEL agents were obtained from Solarbio Co., Ltd.
(Beijing, China). Cell culture agents were purchased from GIBCO. Antibodies
were acquired from Abcam Co., Ltd. (Shanghai, China).

Yu Y., Feng Z., Liu J., Hou X., Zhou X., Gao J., Wang W., Zhang Y., Li G, & Liu J. (2021). γ-Ray-Triggered Drug Release of Reactive Oxygen Species-Sensitive Nanomedicine for Enhanced Concurrent Chemoradiation Therapy. ACS Omega, 6(30), 19445-19457.

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Synthesis and Characterization of Multifunctional Nanomaterials

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Dimethylsulfoxide (DMSO), tetrahydrofuran (THF), dimethyl formamide (DMF) was purchased from China National Medicines Co. Ltd (Shanghai, China). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinimidyl(polyethylene-glycol)-2000] (NHS-PEG-DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N [methoxy(polyethylene glycol)-2000] (PEG-DSPE) were purchased from Xi'an Ruixi Biological Technology Co. Ltd (Xi'an, China). The peptide TMTP1 was purchased from GL Biochem Ltd (Shanghai, China). PVDF membrane was purchased from Millipore Corporation (Bedford, MA, USA). Fetal bovine serum was purchased from Gibco Company (Grand Island, NY, USA). 2′,7′-dichlorofluorescin diacetate (DCFH-DA) and Rose Bengal (RB) were provided by Yeasen Co. Ltd. (Shanghai, China). 9,10-Anthracenediyl-bis(methylene)dim alonic acid (ABDA) and 5-chloromethylfluorescein diacetate (CMFDA) were provided by Sigma-Aldrich (St Louis, MO, USA). Cell Counting Kit-8 (CCK8) was purchased from Beyotime Biotechnology (Shanghai, China).

Dai J., Xu M., Wang Q., Yang J., Zhang J., Cui P., Wang W., Lou X., Xia F, & Wang S. (2020). Cooperation therapy between anti-growth by photodynamic-AIEgens and anti-metastasis by small molecule inhibitors in ovarian cancer. Theranostics, 10(5), 2385-2398.

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