Ten microliters of alendronate sodium trihydrate (0.9 µg/µl) (Sigma, A4978), recombinant mouse osteoprotegerin (rOPG) protein (200 ng/µl) (R&D Systems, Inc., 459-MO-100), or recombinant mouse RANK Ligand (rRANKL) protein (40 ng/µl) (Sigma, R0525), were injected into the vitelline vein of quail and duck at HH33 using glass needles (diameter 0.5 mm, Sutter Instruments Co.), and a PV830 Pneumatic Picopump (World Precision Instruments, Sarasota, FL). Concentrations were determined following dose-response studies and published literature. Five microliters of MMP13 inhibitor (2 µg/µl) (EMD Millipore, Inc., 444283) were administered onto the chorioallantoic membrane of HH33 embryos directly above the head (this approach was used instead of vitelline vein injections, which caused embryonic lethality). Control embryos were treated with 0.1% bovine serum albumin (BSA) or dimethylsulfoxide (DMSO). Embryos were collected at HH38 in PFA.
Glass needles
Manufactured by Sutter Instruments
Glass needles are laboratory tools used for precise fluid manipulation and sample handling. They are made of glass and designed for delicate and controlled liquid transfer applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using glass needles
1
Embryonic Bone Development Modulation
2
Embryonic Bone Development Modulation
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Ten microliters of alendronate sodium trihydrate (0.9 µg/µl) (Sigma, A4978), recombinant mouse osteoprotegerin (rOPG) protein (200 ng/µl) (R&D Systems, Inc., 459-MO-100), or recombinant mouse RANK Ligand (rRANKL) protein (40 ng/µl) (Sigma, R0525), were injected into the vitelline vein of quail and duck at HH33 using glass needles (diameter 0.5 mm, Sutter Instruments Co.), and a PV830 Pneumatic Picopump (World Precision Instruments, Sarasota, FL). Concentrations were determined following dose-response studies and published literature. Five microliters of MMP13 inhibitor (2 µg/µl) (EMD Millipore, Inc., 444283) were administered onto the chorioallantoic membrane of HH33 embryos directly above the head (this approach was used instead of vitelline vein injections, which caused embryonic lethality). Control embryos were treated with 0.1% bovine serum albumin (BSA) or dimethylsulfoxide (DMSO). Embryos were collected at HH38 in PFA.
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