Potato dextrose agar plates pda

F. oxysporum f.sp. lycopersici wild type strain 4287 was originally obtained from J. Tello, University of Almeria, Spain and stored as a glycerol microconidial suspension at −80 C. The pathogenicity of this wild type isolate on tomato plants is routinely confirmed by plant infection assays [24] (link). Fusarium conidia were cultured in potato dextrose broth at 28°C and 150 rpm for 4 days. For preparation of challenge inocula, microconidia were isolated by filtration as described previously [24] (link), collected by centrifugation, washed, and resuspended in sterile physiological saline. The conidial concentration was adjusted with a hemocytometer to the desired density. The actual inoculum level was confirmed by plating serial dilutions on potato dextrose agar plates (PDA; Sigma) and incubating for 24 h at 28°C.

In 2015, five wine regions in Hungary (i.e., Eger, Neszmély, Pécs, Szekszárd, and Villány) were monitored for grapevines exhibiting symptoms of trunk diseases, and then the wood-colonizing fungi were isolated. Three thin discs were cut from the trunks. The traditional isolation protocol was carried out according to Váczy et al. [23 ]: The discs were surface-sterilized in 1% chloramine B solution for 5 min after the bark tissues were removed. The samples were rinsed in sterile distilled water and dried. Then, five wood chips were cut and placed on potato dextrose agar plates (PDA; Sigma-Aldrich, Germany). The plates were incubated at room temperature (21 ± 2°C) and the emerging mycelia were transferred to new PDA plates to obtain pure cultures for morphological and molecular works. In this study, three K. longispora strains, namely, CBS 582.83 (ex-type culture), CBS 824.84, and T15142, from Hungary were examined.