The animal preparation was transferred to the stage of an Olympus intravital microscope (Model BX51WI, Center Valley, PA) linked to a charge-coupled device (CCD) Olympus video camera (Model DP71). Microvessels were visualized using a 20× water-immersion objective. Video images were viewed on a high-resolution color monitor and digitally recorded via DP Controller (Olympus, Center Valley, PA) for off-line analysis (Image J) [24 (link),41 (link)].
Model bx51wi
Manufactured by Olympus
The Olympus BX51WI is a motorized upright microscope designed for advanced research and imaging applications. It features a wide range of capabilities, including fluorescence, phase contrast, and Nomarski differential interference contrast (DIC) imaging. The BX51WI is equipped with a high-quality optical system and motorized components for precise control and automated operation.
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Lab products found in correlation
3 protocols using model bx51wi
1
Intravital Microscopy of Microvessels
2
Imaging Calcium Dynamics in Ovary Epithelium
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Group-raised females of the genotype OAMB-Gal4/UAS-GCaMP6S (oviduct epithelium) or 109-53-Gal4 > UAS-GCaMP6S (ovary epithelium) were reared at 28 °C after eclosion until day 5. We used an imaging setup consisting of a Polychrome V monochromator (TILL Photonics/FEI) as light source and a TILL Imago charge-coupled device (CCD) camera. The microscope (Olympus Model BX51WI) was equipped with a 40X W NA 0.8 objective. Binning on the chip (8 × 8) was set to give a spatial sampling rate of 1 μm/pixel (image size 172 × 130 pixels, corresponding to 172 and 130 μm). Images were taken at a rate of 4 Hz. The excitation wavelength was 488 nm, and the exposure time was 25 ms. Fluorescent light passing an excitation filter (370–510 nm) was directed onto a 500-nm DCLP mirror followed by a 515 LP emission filter for enhanced green fluorescent protein. Timing of treatment was performed as above; the bath was 180 μl and 20 μl for treatment as indicated.
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Intravital Microscopy of Microvessels
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