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αb220 apc

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αB220-APC is a monoclonal antibody that binds to the B220 (CD45R) antigen. B220 is expressed on the surface of B cells and is commonly used as a marker to identify and study B cell populations. The αB220-APC conjugate allows for the detection and analysis of B cells by flow cytometry.

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Lab products found in correlation

Facscanto analyzer, by BD (2 mentions) αpd 1, by Santa Cruz Biotechnology (2 mentions) Abd pe, by Santa Cruz Biotechnology (2 mentions) αcd4 pe, by BioLegend (2 mentions) αcd8α pe, by Santa Cruz Biotechnology (2 mentions) α b220 pe, by BD (1 mentions) α igm fitc, by BD (1 mentions) α cd3 pe, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Lsrfortessa, by BD (1 mentions)

3 protocols using αb220 apc

1

Immunomodulation of NOD and C57BL6 Mice

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Ten-week-old female NOD mice were randomly assigned into 4 groups and treated with one dose of αPD-1-ABD-PE (5 mg/kg), a mixture of αPD-1 (scFv, 2.5 mg/kg) and ABD-PE (2.5 mg/kg), PBS, or CP (200 mg/kg) (Santa Cruz Biotechnology) intraperitoneally. These mice were euthanized 24 hours later. Blood samples and splenocytes were collected from the mice, and red blood cells in these samples were lysed with the Ammonium-Chloride-Potassium (ACK) Lysing Buffer. The remaining cells in the samples were pelleted by a centrifugation at 300 g for 5 minutes, and stained by αB220-APC (Biolegend), αCD4-PE (Biolegend), or αCD8α-PE (Santa Cruz Biotechnology, Inc). The B220+, CD4+, and CD8+ fractions in the pellets were analyzed by flow cytometry on a BD FACSCanto Analyzer. The same experiment was conducted on 10-week-old female C57BL6 mice.
Zhao P., Wang P., Dong S., Zhou Z., Cao Y., Yagita H., He X., Zheng S.G., Fisher S.J., Fujinami R.S, & Chen M. (2019). Depletion of PD-1-positive cells ameliorates autoimmune disease. Nature biomedical engineering, 3(4), 292-305.
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2

Immunomodulation of NOD and C57BL6 Mice

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Ten-week-old female NOD mice were randomly assigned into 4 groups and treated with one dose of αPD-1-ABD-PE (5 mg/kg), a mixture of αPD-1 (scFv, 2.5 mg/kg) and ABD-PE (2.5 mg/kg), PBS, or CP (200 mg/kg) (Santa Cruz Biotechnology) intraperitoneally. These mice were euthanized 24 hours later. Blood samples and splenocytes were collected from the mice, and red blood cells in these samples were lysed with the Ammonium-Chloride-Potassium (ACK) Lysing Buffer. The remaining cells in the samples were pelleted by a centrifugation at 300 g for 5 minutes, and stained by αB220-APC (Biolegend), αCD4-PE (Biolegend), or αCD8α-PE (Santa Cruz Biotechnology, Inc). The B220+, CD4+, and CD8+ fractions in the pellets were analyzed by flow cytometry on a BD FACSCanto Analyzer. The same experiment was conducted on 10-week-old female C57BL6 mice.
Zhao P., Wang P., Dong S., Zhou Z., Cao Y., Yagita H., He X., Zheng S.G., Fisher S.J., Fujinami R.S, & Chen M. (2019). Depletion of PD-1-positive cells ameliorates autoimmune disease. Nature biomedical engineering, 3(4), 292-305.
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3

Phenotypic Analysis of Activated B Cells

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For survival and class switch recombination experiments, mouse spleens of RD/RDF WT and KO mice were isolated and single B cell suspensions of the whole tissue were prepared after MACS depletion as described above. Activated B cells were stained with α-B220-BV785 (103246; BioLegend), α-IgG1-FITC (553443; BD) and TO-PRO3 (Invitrogen). CSFE cultivated, activated B cells of RD mice were stained with α-B220-APC (103212; BioLegend) and DAPI (Sigma).
Mouse spleens of RDM/RDFM WT and KO mice were isolated and cell suspensions of the whole tissue were prepared. Cells were stained with α-CD3-PE (553064; BD), α-B220-BV785, α-B220-PE (553090, BD Biosciences), α-CD19-BV421 (115,549; BioLegend), α-IgM-FITC (553408; BD Biosciences), α-CD138-Pe-Cy7 (142514; BioLegend), Sca-1 (or Ly-6A7E; 108139; BioLegend), and TO-PRO3 or DAPI . Flow cytometry was performed on a LSR Fortessa (BD Biosciences) and analysed in FlowJo (FlowJo, LLC).
Kalweit K., Gölling V., Kosan C, & Jungnickel B. (2024). Role of Rad18 in B cell activation and lymphomagenesis. Scientific Reports, 14, 7066.
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