Elisa kit for pge2

Cell supernatants were harvested and PG concentrations were quantified using an ELISA kit for PGE₂ (Enzo Life Sciences), according to the manufacturer’s instructions. This item is sold as a PGE₂-specific ELISA kit, however, our data indicated that other COX-derived oxylipins were also likely to be detected with this kit. Therefore we refer to results obtained using this ELISA kit as measuring PGs instead of PGE₂.

The human subject samples were collected as part of approved Institutional Review Board protocols (STU 082010‐016, MR 17‐120). Patient samples were collected during their visit to UTSW Medical Center and written consent was obtained before sample collection and participation in the study. As a proof of concept, three postmenopausal female subjects with active, symptomatic UTIs as diagnosed by clinical urine culture were studied. ELISA kit for PGE2 was purchased from Enzo Life Sciences Inc. (Farmingdale, NY, USA), IL‐6 was purchased from Abcam (Cambridge, MA, USA), and that for CRP was purchased from Sigma Aldrich (St. Louis, MO, USA). Clean‐catch urine collected from these patients was aliquoted and stored at −20 C and was retrieved later for analysis. Postanalysis was done by using the calibration dose–response curves for both methods described in the previous sections. Graphics were drawn using Biorender.comand Inkscape v1.