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Recombinant human pdx 1

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Recombinant human PDX-1 is a protein produced using recombinant DNA technology. PDX-1 is a transcription factor that plays a key role in the development and function of pancreatic beta cells.

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Lab products found in correlation

32p atp, by PerkinElmer (2 mentions)

2 protocols using recombinant human pdx 1

1

PDX1 Phosphorylation by MST1 Kinase

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Purified human active MST1 (Upstate Biotechnology) with or without GST tag was incubated with 32P-ATP (2 μCi, Perkin Elmer Life Sciences), ATP (100 μM) and 1 mM dithiothreitol (DTT) in a kinase buffer containing 40 mM HEPES (pH 7.4), 20 mM MgCl2, 1mM EDTA and 1 μg of purified recombinant human PDX-1 (Abcam) or bacterially purified GST-PDX1 (WT and mutants) as substrates. After incubation at 30°C for 30 min, the reaction was stopped by adding loading buffer and proteins were separated on NuPAGE gels and phosphorylation levels visualized either by autoradiography or specific antibody for phospho-PDX1. The total PDX1 was detected with anti-PDX1 antibody.
Ardestani A., Paroni F., Azizi Z., Kaur S., Khobragade V., Yuan T., Frogne T., Tao W., Oberholzer J., Pattou F., Conte J.K, & Maedler K. (2014). MST1 is a novel regulator of apoptosis in pancreatic beta-cells. Nature medicine, 20(4), 385-397.
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2

PDX1 Phosphorylation by MST1 Kinase

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified human active MST1 (Upstate Biotechnology) with or without GST tag was incubated with 32P-ATP (2 μCi, Perkin Elmer Life Sciences), ATP (100 μM) and 1 mM dithiothreitol (DTT) in a kinase buffer containing 40 mM HEPES (pH 7.4), 20 mM MgCl2, 1mM EDTA and 1 μg of purified recombinant human PDX-1 (Abcam) or bacterially purified GST-PDX1 (WT and mutants) as substrates. After incubation at 30°C for 30 min, the reaction was stopped by adding loading buffer and proteins were separated on NuPAGE gels and phosphorylation levels visualized either by autoradiography or specific antibody for phospho-PDX1. The total PDX1 was detected with anti-PDX1 antibody.
Ardestani A., Paroni F., Azizi Z., Kaur S., Khobragade V., Yuan T., Frogne T., Tao W., Oberholzer J., Pattou F., Conte J.K, & Maedler K. (2014). MST1 is a novel regulator of apoptosis in pancreatic beta-cells. Nature medicine, 20(4), 385-397.
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