Unify software 1

Every phenolic compound in the leaf extract of C. olitorius at the optimized microwave-enhanced extraction conditions was identified using LC-QToF-MS (Waters Vion IMS, USA). A C₁₈ column with the dimension of 100 mm × 2.10 mm × 1.80 µm was used. There are two mobile phases used in this analysis; they are water + 0.1% formic acid (A) and 100% acetonitrile (B). The operating conditions were a seal wash period of 5 min, a pre-injector volume of 0 µL, low pressure of 0 psi, high-pressure limit of 18,000 psi, gradient elution was 90% A and 10% B (1.25 min), 45% A and 55% B (4.17 min), 10% A and 90% B (6.25 min), 90% A and 10% B (8.34 min), with the injection volume of 20 µL, the flow rate of 0.50 mL/min, spray voltage of 4 keV, mass range of 100–1000 m/z, sample temperature of 15 °C, column temperature of 40 °C, gas flow of 0.50 mL/min, desolvation gas flow rate of 800 L/h, desolvation temperature of 550 °C, low collision energy of 4 eV, high collision energy ramp between 10 and 45 eV, source temperature of 120 °C. The identifications of phenolic compounds in the leaf extract of C. olitorius were performed by utilising SYNAPT mass spectrometer (Waters) coupled with an electrospray ionization operated in negative ion mode. Waters® UNIFY Software 1.0.0 installed in equipment was used to identify the phenolic compounds.