B6.129s cd14tm1frm j

T-cell/B-cell-deficient SCID (B6; 129S7-Rag1^tm1mom/J), TNF-deficient (B6;129S-Tnf^tm1Gkl/J), TNF receptor 1 (TNFR1)-deficient (B6;129S-Tnfrsf1a^tm1Imx Il1r1^tm1Imx/J), TNFR2-deficient (B6.129S2-Tnfrsf1b^tm1Mwm/J); CD14-deficient (B6.129S-Cd14^tm1Frm/J) and TLR4-deficient (C.C3-Tlr4^Lps-d/J) female mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Mice were housed at the University of Pittsburgh Cancer Institute’s Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) internationally accredited animal facility. The animal studies were performed in accordance with protocols approved by the University of Pittsburgh Institutional Animal Care and Use Committee.

Eight- to 12-wk-old male wild type (WT) C57BL/10SNJ mice, Tlr4 knock out (KO) mice (C57BL/10ScNJ), WT mice (C57BL/6J), and Cd14KO mice (B6.129S-Cd14tm1Frm/J) were purchased from (Jackson Laboratory, Bar Harbor, ME) for these studies. The mice were maintained in a pathogen-free environment at the University of Texas Medical Branch (Galveston, TX). Animal experiments were performed according to the NIH Guide and were approved by the UTMB Animal Care and Use Committee.