Venous blood samples were collected in the morning after fasting overnight. An auto-biochemical analyzer (Roche Diagnostic GmbH) was used to determine the fasting blood glucose (FBG) and serum concentrations of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), serum uric acid (UA), fasting plasma insulin, and C-reactive protein (CRP). Glycated hemoglobin (HbA1c) levels were quantified using high-performance liquid chromatography. Serum total osteocalcin level was measured by electrical chemiluminescent immunoassay using a modular E170 analyser (Roche Diagnostics).
E170 analyser
Manufactured by Roche
Sourced in United Kingdom
The E170 analyzer is a compact and automated biochemistry analyzer designed for clinical laboratories. It is capable of performing a wide range of routine and specialized clinical chemistry tests. The E170 analyzer utilizes advanced spectrophotometric technology to provide accurate and reliable test results.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using e170 analyser
1
Comprehensive Metabolic Profiling of Fasting Samples
2
Assessing Cardiovascular Biomarkers
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Venous blood samples were collected for haematology, biochemistry, and lipid profiles. Serum N-terminal pro B natriuretic peptide (NT-proBNP, ng/L) was measured by sandwich immunoassay with magnetic particle separation and chemiluminescent detection on an E170 analyser (Roche Diagnostics, Burgess Hill, United Kingdom) with a lower limit of detection of 0.6 pmol/L. Dyslipidaemia was defined as fasting cholesterol > 5 mmol/L and/or triglycerides > 1.7 mmol/L.
3
Biochemical Markers of Cardiovascular Health
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Venous blood samples were collected for routine haematology, biochemistry and lipid profiles. Serum N-terminal pro B Natriuretic peptide (NT-proBNP, ng/L) was measured by sandwich immunoassay with magnetic particle separation and chemiluminescent detection on an E170 analyser (Roche Diagnostics, Burgess Hill) with a lower limit of detection of 0.6 pmol/L. Plasma renin was measured using an immunoradiometric assay (Cis Bio, IBA Molecular UK, Guildford) with age and position reference intervals and a detection limit of 5 ng/L. Aldosterone was measured by competitive radioimmunoassay (Coat-a-Count, Siemens Medical Solutions Diagnositics, Llanberis.) which has reference intervals dependent on position with a detection limit of 60 pmol/L. C-peptide (pmol/L)/insulin (pmol/L) were also measured (Royal Surrey County Hospital, Guildford).
4
Venous Blood Sample Processing and Biomarker Measurement
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Venous blood samples were collected and transported as whole blood using a fast transport service (TNT company) from recruiting centres to Genethon (Evry, France).
Samples were immediately centrifuged. Supernatant serum was stored at -80°C until the samples were analyzed in the Biochemistry Department of Pitie-Salpetriere Hospital, Paris. Serum NT-proBNP was measured by a two-site electrochemiluminescence immunoassay on a Roche Diagnostics E170 analyser. The limit of quantitation is <50 pg/ml, and the coefficients of variation of intra-and interassay reproducibility are <5%. MR-proANP was measured by a sandwich chemiluminescence immunoassay on the KRYPTOR system (BRAHMS GmbH, Hennigsdorf, Germany). The limit of quantitation is 4.5 pmol/l, the coefficient of variation of intra-assay reproducibility was <5% and the coefficient of variation of inter-assay reproducibility was <6.5 %. (13) Follow-up Patients were followed in their local centres for a median observation time of 23 months. The investigator recorded the following endpoints during a clinic visit or by telephone: death, heart failure hospitalisation, cardiac transplantation or left ventricular assist device, and also intracardiac defibrillator implantation and appropriate shocks, septal myectomy and alcohol septal ablation.
Samples were immediately centrifuged. Supernatant serum was stored at -80°C until the samples were analyzed in the Biochemistry Department of Pitie-Salpetriere Hospital, Paris. Serum NT-proBNP was measured by a two-site electrochemiluminescence immunoassay on a Roche Diagnostics E170 analyser. The limit of quantitation is <50 pg/ml, and the coefficients of variation of intra-and interassay reproducibility are <5%. MR-proANP was measured by a sandwich chemiluminescence immunoassay on the KRYPTOR system (BRAHMS GmbH, Hennigsdorf, Germany). The limit of quantitation is 4.5 pmol/l, the coefficient of variation of intra-assay reproducibility was <5% and the coefficient of variation of inter-assay reproducibility was <6.5 %. (13) Follow-up Patients were followed in their local centres for a median observation time of 23 months. The investigator recorded the following endpoints during a clinic visit or by telephone: death, heart failure hospitalisation, cardiac transplantation or left ventricular assist device, and also intracardiac defibrillator implantation and appropriate shocks, septal myectomy and alcohol septal ablation.
5
Lipid and Enzyme Profiling in Metabolic Assessment
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Triacylglycerol was measured by quantitative determination with glycerol blanking. HDL-cholesterol was measured directly using polyethylene glycol-modified enzymes and dextran sulphate. When cholesterol esterase and cholesterol oxidase enzymes are modified by polyethylene glycol, they show selective catalytic activity towards lipoprotein fractions, with reactivity increasing in the order LDL < VLDL ≈ chylomicrons < HDL. Total cholesterol was measured by an enzymatic, colorimetric method. LDL-cholesterol was calculated from the total cholesterol, HDL-cholesterol and triacylglycerol concentrations using the Friedewald formula: LDL = total cholesterol – HDL-cholesterol – (triacylglycerol/2.2). ALT and AST were measured by UV absorbance without pyridoxal phosphate activation. ALT, AST, cholesterol, glucose, triacylglycerol and HDL-cholesterol were measured using a Roche MODULAR P analyser (Roche Diagnostics, Indianapolis, IN, USA). Insulin and C-peptide were measured using a Roche E170 analyser (Roche Diagnostics).
6
Home Urine C-Peptide Ratio Protocol
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