Soya phosphatidylcholine spc

RPMI-1640, alpha-MEM media and the normal human dermal fibroblast cell line (NHDF) were purchased from Lonza (Lonza, Warsaw, Poland), while fetal bovine serum (FBS) was from Biomedia (EuroClone by Biomedica, Piaseczno, Poland), GlutaMAX™ (L-alanyl-L-glutamine dipeptide in 0.85% NaCl) and 100× antibiotic-antimycotic were purchased from Life Technologies (Gibco/Life Technologies, Warsaw, Poland). The BxPC-3 cell line was purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA). The AsPC-1 cell line was kindly provided by the Institute of Immunology and Experimental Therapy (Wroclaw, Poland). Nile Red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one), DAPI (2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were from Sigma-Aldrich (Poznan, Poland), chloroform, methanol and DMSO from Archem (Kamieniec Wroclawski, Poland). Soya phosphatidylcholine (SPC) was purchased from Lipoid GmbH (Ludwigshafen, Germany). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG₂₀₀₀) was obtained from Northern Lipids (Vancouver, BC, Canada).

DTX was purchased from Wuhan Xinxin Jiali Biological Technology Co, Ltd (Wuhan, China). RGD, Lf, and coumarin-6 (C6, purity > 98%) were obtained from Sigma Aldrich (St. Louis, USA). Cholesterol (Chol) was purchased from Shanghai Xingzhi Chemical Factory Co, Ltd (Shanghai, China). Soya phosphatidyl choline (SPC) was obtained from Lipoid GmbH (Ludwigshafen, Germany). The DSPE-PEG2000 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N- [methoxy(polyethyleneglycol)-2000]), DSPE-PEG₂₀₀₀-maleimide (DSPE-PEG₂₀₀₀-Mal), and DSPE-PEG₂₀₀₀-RGD were purchased from Ponsure Co, Ltd (Shanghai, China). 2-iminothiohne (Traut’s) was purchased from Thermo Fischer Scientific (Massachusetts, USA). Sephadex G-50, Sepharose CL-4B, Trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and TritonX-100 were purchased from Solarbio Co, Ltd (Beijing, China). Hoechst 33342 and DiR dye were purchased from YEASEN Co, Ltd (Shanghai, China). DMEM medium was obtained from Gibco (California, USA). Fetal bovine serum (FBS) was purchased from GEMINI (California, USA). The high-performance liquid chromatography (HPLC)-grade solvents used for HPLC were purchased from Xilong Chemical Co, Ltd. (Shantou, China). All other chemicals utilized were analytical grade preparations.

Soya phosphatidylcholine (SPC) was obtained from the Lipoid (Steinhausen, Switzerland), under the brand name of Phospholipon 90. The suppliers of other reagents were: PG (Chempure, Singapore), dialysis tubing (Thermo Scientific, Waltham, MA, USA), ibuprofen and ibuprofen sodium (Sigma, Singapore), tofacitinib citrate (Sigma, Singapore), rhodamine B (Sigma, Singapore), calcein (Sigma, Singapore), lidocaine (Tokyo Kasei, Tokyo, Japan), phosphate buffered saline (PBS) (Vivantis, Singapore). Polyethylene glycol-4-arm, succinimidyl glutarate, bovine fibrinogen, bovine thrombin and calcium chloride were purchased from Sigma-Aldrich, Singapore. Growth medium for culturing keratinocytes, fibroblasts, pericytes and human dermal microvascular cells were purchased from PromoCell, Heidelberg, Germany. ThinCert culture ware and tissue culture flasks were purchased from Greiner Bio-One, Kremsmünster, Austria. Water was purified by using Milli-Q system (Millipore, Billerica, MA, USA).