Phytohaemagglutinin p

Manufactured by Merck Group

Sourced in United States

Phytohaemagglutinin P is a laboratory reagent derived from the red kidney bean (Phaseolus vulgaris). It functions as a mitogenic lectin, stimulating the proliferation of T lymphocytes in cell culture. This property makes it a useful tool for research in immunology and cell biology.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using phytohaemagglutinin p

Comprehensive Analysis of Canine Gut and Immune Status

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ground samples of food and faeces were analysed for DM, organic matter, crude protein, ether extract, crude fibre and crude ash, while N-free extract was calculated⁽¹² ⁾. The pH of the faecal samples was determined by a pH meter (Eutech Instruments). Lactate, ammonia, SCFA and branched-chain fatty acid (BCFA) concentrations in the faecal samples were analysed as described earlier⁽¹³ (link)⁾.
Cell-mediated immunity (CMI) was assessed at 8 weeks by measurement of skin indurations as type-IV delayed-type hypersensitivity (DTH) reaction to phytohaemagglutinin-P (Sigma) as a mitogen, as detailed previously⁽¹⁴ ⁾. For the humoral immune response, dogs were intravenously injected with 1 ml of a 10 % suspension of washed sheep erythrocytes after 5 weeks of experimental feeding. Serum samples collected at periodic intervals (0, 7, 14, 21 and 28 d) were used for an antibody titre assay using the microtitre haemagglutination procedure⁽¹⁵ ⁾.

Kumar S., Pattanaik A.K., Sharma S., Gupta R., Jadhav S.E, & Dutta N. (2017). Comparative assessment of canine-origin Lactobacillus johnsonii CPN23 and dairy-origin Lactobacillus acidophillus NCDC 15 for nutrient digestibility, faecal fermentative metabolites and selected gut health indices in dogs. Journal of Nutritional Science, 6, e38.

+ Open protocol

+ Expand

Tracking γδ T Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

RPMI-1640, FBS, non-essential amino acids, pyruvate, penicillin streptomycin, HEPES, 2-ME, HMBPP and FITC-γδ TCR antibody (clone 5A6.E91) were obtained through Fisher Scientific. IL-2 and the MACS γδ T cell negative selection kit were obtained through Miltenyi. PE-CD3 (UCHT1), APC-TNFα (MAb11), PE-IFNγ (4S.B3), FITC-IL-2 (MQ1-17H12), APC-CD45RA (HI-100), PerCP-eFluor710-CD27 (LG.7F9), FITC-Annexin V and PerCP-eFluor710-Annexin V were from eBioscience. K562 cells were from ATCC while Research Blood Components (Boston, MA) supplied blood. DiD, calcein AM, pcDNA3.1 and DNA primers were obtained through Life Technologies, while CRISPR/Cas9 repair templates were obtained through IDT. Restriction enzymes including XhoI, EcoRV and NheI were obtained through New England Biolabs. PE-BTN3A1 (CD277) antibody (BT3.1), FITC-CD183 (G025H7), functional grade anti-human TCR γ/δ Antibody (B1), and human IFNγ ELISA MAX deluxe kit were obtained from Biolegend while G418, PP2 and phytohaemagglutinin P were obtained from Sigma. Anti-myc (9E10) antibody was purified from hybridoma cells obtained from the Developmental Studies Hybridoma Bank at The University of Iowa. POM₂-C-HMBP was a kind gift of Dr. David Wiemer at the University of Iowa.

Kilcollins A.M., Li J., Hsiao C.H, & Wiemer A.J. (2016). HMBPP analog prodrugs bypass energy-dependent uptake to promote efficient BTN3A1-mediated malignant cell lysis by Vγ9Vδ2 T lymphocyte effectors. Journal of immunology (Baltimore, Md. : 1950), 197(2), 419-428.

+ Open protocol

+ Expand

Isolation and Culture of Avian Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were collected from adult mallard ducks and white Chinese geese (reared in Sapporo Maruyama Zoo, Sapporo, Hokkaido, Japan) in heparinized blood collection tubes to prevent blood coagulation. Blood samples were centrifuged at 400 Xg for 15 min at room temperature (RT), pellets were suspended in 10 ml of phosphate-buffered saline (PBS), and then, carefully layered on 3.0 ml of HISTOPAQUE-1077 (Sigma-Aldrich, St. Louis, MO, USA) followed by centrifugation at 400 Xg for 30 min at RT. The opaque interface layer containing leukocytes was collected and transferred into a new tube, washed twice with 10 ml of PBS, and then centrifuged at 250 Xg for 10 min at RT. Thereafter, the leukocytes were suspended and cultured in 10 ml of RPMI 1640 medium (Gibco/Invitrogen, Carlsbad, CA, USA) containing 1% penicillin/streptomycin/glutamine (Gibco/Invitrogen), 10% fetal bovine serum (FBS) (Atlas Biologicals, Inc. Fort Collins, CO, USA), 1μg/ml phytohaemagglutinin P (Sigma-Aldrich), and 10 μg/ml pokeweed mitogen (Sigma-Aldrich) in a 5% CO2 incubator at 37 °C for 48 h.

Tag-El-Din-Hassan H.T., Morimatsu M, & Agui T. (2018). Functional analysis of duck, goose, and ostrich 2'-5'-oligoadenylate synthetase. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 62.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!