Trifluoroacetic acid tfa

Manufactured by AAPPTec

Trifluoroacetic acid (TFA) is a colorless, corrosive liquid with a pungent odor. It is a strong organic acid commonly used in various laboratory applications, including peptide synthesis, chromatography, and organic synthesis.

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Lab products found in correlation

Varioskan flash, by Thermo Fisher Scientific (1 mentions) Pegda, by Merck Group (1 mentions) Triethanolamine tea, by Merck Group (1 mentions) Span 80, by Merck Group (1 mentions) Sodium hexametaphosphate crystalline, by Merck Group (1 mentions) 2 2 azobis 2 methylpropionamidine dihydrochloride v 50, by Thermo Fisher Scientific (1 mentions) Acryl peg sva, by Laysan Bio (1 mentions) Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions) Tween 20, by Merck Group (1 mentions) Acetone, by Thermo Fisher Scientific (1 mentions) Diethyl ether, by Thermo Fisher Scientific (1 mentions) Piperidine, by Merck Group (1 mentions) Potassium persulfate, by Merck Group (1 mentions) Eosin y, by Merck Group (1 mentions) Ultra high purity nitrogen, by Airgas (1 mentions) N n diisopropylethylamine diea, by Thermo Fisher Scientific (1 mentions)

2 protocols using trifluoroacetic acid tfa

Fluorescence Assay for Carnosine Methyl-Ester

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All solvents and reagents, unless otherwise specified, were purchased from Sigma-Aldrich. Sodium hyaluronate (200 and 700 kDa) was a kind gift from Fidia Farmaceutici S.p. A (IT). 3-Hydroxy,1,2,3-benzotriazin-4(3H)-one (HOOBt) and trifluoroacetic acid (TFA) were purchased from AAPPTec and VWR, respectively. 3-(N-morpholino)-propanesulphonic acid (MOPS) buffer solution (1 mM, pH 7.4, supplemented with 0.003 M KCl and 0.14 M NaCl) was prepared from a powder (Amresco Biochemicals).
Anhydrous methanol was kept for 24 h on 4 Å molecular sieves before use. Carnosine methyl-ester (Car-OMe) was synthesized in anhydrous methanol, as previously reported^{94 (link)}.
All the fluorescence and UV–Vis spectra were reordered by using a multimode microplate reader (Varioskan Flash, Thermo Scientific).

Greco V., Naletova I., Ahmed I.M., Vaccaro S., Messina L., La Mendola D., Bellia F., Sciuto S., Satriano C, & Rizzarelli E. (2020). Hyaluronan-carnosine conjugates inhibit Aβ aggregation and toxicity. Scientific Reports, 10, 15998.

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Synthesis and Characterization of PEG-based Hydrogels

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Acryl-PEG-SVA (MW= 5,000 g/mol) was purchased from Laysan Bio (Arab, AL). PEGDA (MW= 575 g/mol), Vinyl-2-pyrrolidone (NVP), potassium persulfate (KPS), sodium hexametaphosphate crystalline, Span® 80, Tween® 20, Eosin Y, triethanolamine (TEA), fluorescein isothiocyanate (FITC), 2,2′-(Ethylenedioxy)bis(ethylamine) (EDEA) and piperidine were purchased from Sigma Aldrich (St Louis, MO). Cyclohexane 99%, potassium phosphate monobasic crystals and 2,2’-Azobis(2-methylpropionamidine) dihydrochloride (V-50) were purchased from Acros Organics. Acetone, 1-Ethyl-3-(3-Dimethylaminopropyl)carbodiimide (EDAC), triisopropylsilane, diethyl ether and N,N-diisopropylethylamine (DIEA), were purchased from Fisher Scientific (Hanover Park, IL). All amino acids, resin, O-benzotriazole-N,N,N’,N’-tetramethyluronium-hexafluoro-phosphate (HBTU), N,N-dimethylformamide (DMF) and trifluoroacetic acid (TFA) were purchased from AAPPTec (Louisville, KY). Please add Pi and PPi names and vendor information here. Ultra-high purity (UHP) nitrogen was purchased from Airgas (Radnor, PA, USA). All the other reagents were used without further processing.

Pimentel M.B., Borges F.T., Teymour F., Zaborina O.Y., Alverdy J.C., Fang K., Hong S.H., Staneviciute A., He Y.J, & Papavasiliou G. (2020). An in vitro tissue model for screening sustained release of phosphate-based therapeutics in attenuating pathogen-induced proteolytic matrix degradation. Journal of materials chemistry. B, 8(12), 2454-2465.

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