Homer1b c

Manufactured by Synaptic Systems

Homer1b/c is a laboratory equipment product that serves a core function. The description of this product is maintained in a concise, unbiased, and factual manner without extrapolation on its intended use.

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Lab products found in correlation

Vglut1, by Synaptic Systems (2 mentions) Nlgn2, by Synaptic Systems (1 mentions) Prolong gold antifade reagent with dapi, by Cell Signaling Technology (1 mentions) Vectashield antifade mounting medium with dapi, by Vector Laboratories (1 mentions) Gad65, by Abcam (1 mentions) Ctip2, by Abcam (1 mentions) Glun1, by Merck Group (1 mentions) Bassoon, by Enzo Life Sciences (1 mentions) Vglut2, by Synaptic Systems (1 mentions) Anti ubiquitin, by Abcam (1 mentions) Anti lc3a, by Cell Signaling Technology (1 mentions) Alexa fluor 647 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Alexa fluor 647 goat anti chicken, by Thermo Fisher Scientific (1 mentions) Anti chat, by Abcam (1 mentions) Anti lamp1, by Thermo Fisher Scientific (1 mentions) Anti synaptophysin, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions)

Spelling variants of this product

Anti-Homer1b/c (2 citations)

3 protocols using homer1b c

Immunofluorescence Analysis of Synaptic Markers

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For immunofluorescence analyses, 4% paraformaldehyde (PFA) fixed coronal or sagittal brain slices (30 μm thick) were processed. After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling, #3724, 1:500 dilution), in combination with one of four primary guinea pig antibodies against synaptic markers: Homer1b/c (Synaptic Systems, #160 025, 1:200), Nlgn2 (Synaptic Systems, #129 205, 1:500), vGluT1 (Synaptic Systems, #135 304, 1:15,000), vGAT (Synaptic Systems, #129 205, 1:500). This was followed by incubation with corresponding Alexa 488 goat anti-guinea pig (Invitrogen, #A-11073, 1:500) and Alexa 594 goat anti-rabbit (Invitrogen, #A-11012, 1:500) secondary antibodies. Slides were mounted with either Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories, # H-1200) or ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, # 8961S).

Bemben M.A., Sandoval M., Le A.A., Won S., Chau V.N., Lauterborn J.C., Incontro S., Li K.H., Burlingame A.L., Roche K.W., Gall C.M., Nicoll R.A, & Diaz-Alonso J. (2023). Contrastsing synaptic roles of MDGA1 and MDGA2. bioRxiv.

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Immunocytochemistry and Western Blot Antibody Validation

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Primary antibodies used for immunocytochemistry were all diluted 1:200, for western blotting a dilution of 1:500 was used (except for Actin which was diluted 1:100000). The Shank2 (“ppI-SAM pabSA5192”) and Shank3 antibodies (“PRC pab,” simply referred to as “Shank3” in the study and “C-term/ProSAP2/Shank3”) have been characterized previously (Bockers et al., 1999 (link); Bockmann et al., 2002 (link); Schmeisser et al., 2012 (link)). The following primary antibodies were purchased from commercial suppliers: Actin (Sigma-Aldrich Cat# A2228 RRID:AB_476697), Bassoon (Enzo Life Sciences Cat# ADI-VAM-PS003-F RRID:AB_11181058), CTIP2 (Abcam Cat# ab18465 RRID:AB_2064130) GAD65 (Abcam Cat# ab85866 RRID:AB_1860505), Homer 1b/c (Synaptic Systems GmbH Cat# 160 023, no RRID yet), GluN1 (Sigma-Aldrich Cat# G8913 RRID:AB_259978), Shank1 (Novus Cat# NB300-167 RRID:AB_2187584), SPO (Synaptic Systems GmbH Cat# 102 002 RRID:AB_887841), Syn1/2 (Synaptic Systems GmbH Cat# 160 003, no RRID yet), VGLUT1 (Synaptic Systems GmbH Cat# 135 304 RRID:AB_887878), VGLUT2 (Synaptic Systems GmbH Cat# 135 404 RRID:AB_887884), and VGLUT 3 (Synaptic Systems Cat# 135204, no RRID yet).

Heise C., Schroeder J.C., Schoen M., Halbedl S., Reim D., Woelfle S., Kreutz M.R., Schmeisser M.J, & Boeckers T.M. (2016). Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus. Frontiers in Cellular Neuroscience, 10, 106.

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Comprehensive Immunostaining Protocol for Neuronal Markers

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In this study, the following primary antibodies were used: anti-MAP2 (Encor, CPCA-MAP2), anti-LC3A (Cell Signaling, 4599), anti-ChAT (custom made by Abcam in rat using the same epitope as ab181023), anti-Synaptophysin (Abcam, ab14692), Homer1 b/c (Synaptic Systems, 160 025), anti-LAMP1 (Invitrogen, MA1-164), anti-cleaved caspase 3 (Cell Signaling, 9661), anti-ubiquitin (Abcam, ab7254), anti-C9orf72 (Genetex, GTX632041, anti-β-actin (Sigma-Aldrich, A5441), anti-RagC (Cell Signaling, 9480), anti-FLCN (Proteintech, 11236-2-AP), anti-NPRL2 (Cell Signaling, 37 344).
For western blot experiments, we used HRP-conjugated anti-Mouse purchased from DAKO.
For immunostainings, the following secondary antibodies from ThermoFisher Scientific were used: goat anti-Chicken DyLight 350 (SA5-10069), goat anti-Mouse Alexa Fluor® 488 (A-11001), goat anti-Rabbit Alexa Fluor® 488 (A-11008), goat anti-Mouse Alexa Fluor® 568 (A-11004), goat anti-Rabbit Alexa Fluor® 568 (A-11011), goat anti-Chicken Alexa Fluor® 647 (A32933), goat anti-Mouse Alexa Fluor® 647 (A-21235), goat anti-Rabbit Alexa Fluor® 647 (A-21244).

Seidel M., Rajkumar S., Steffke C., Noeth V., Agarwal S., Roger K., Lipecka J., Ludolph A., Guerrera C.I., Boeckers T, & Catanese A. (2023). Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models. Current Research in Neurobiology, 5, 100105.

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