Immunofluorescence staining to observe the expression of NETosis markers was performed on formalin-fixed paraffin-embedded (FFPE) tissues utilizing the following primary antibodies: rabbit polyclonal anti-Elane (Atlas) at the dilution of 1:50 (citrate based pH 9.0 epitope retrieval solution); chicken polyclonal anti-Histone H2B (Abcam) at the dilution of 1:500 (citrate based pH 9.0 epitope retrieval solution) rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam) at the dilution of 1:50 (citrate based pH 6.0 epitope retrieval solution). The following were used as secondary antibodies: CyTM2 Affinipure Donkey Anti-rabbit (Jackson Immuno Research) at the dilution of 1:400, CYTM3 Affinipure Donkey Anti-chicken (Jackson Immuno Research) at the dilution of 1:400. The colorations were read and interpreted by using a fluorescence confocal microscope. Immunoreactivity was evaluated by two investigators (LR and AG) and discordant cases were subsequently discussed and agreed upon. Thermo Scientific DAPI Nuclear Counterstains was used for nuclear staining.
Cytm2 affinipure donkey anti rabbit
Manufactured by Jackson ImmunoResearch
CyTM2 Affinipure Donkey Anti-rabbit is a secondary antibody designed for use in immunological applications. It is generated in donkeys and specifically recognizes rabbit immunoglobulins.
Automatically generated - may contain errors
3 protocols using cytm2 affinipure donkey anti rabbit
1
NETosis Marker Expression in FFPE Tissues
2
NETosis Marker Expression in FFPE Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescence staining to observe the expression of NETosis markers was performed on formalin-fixed paraffin-embedded (FFPE) tissues utilizing the following primary antibodies: rabbit polyclonal anti-Elane (Atlas) at the dilution of 1:50 (citrate based pH 9.0 epitope retrieval solution); chicken polyclonal anti-Histone H2B (Abcam) at the dilution of 1:500 (citrate based pH 9.0 epitope retrieval solution) rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam) at the dilution of 1:50 (citrate based pH 6.0 epitope retrieval solution). The following were used as secondary antibodies: CyTM2 Affinipure Donkey Anti-rabbit (Jackson Immuno Research) at the dilution of 1:400, CYTM3 Affinipure Donkey Anti-chicken (Jackson Immuno Research) at the dilution of 1:400. The colorations were read and interpreted by using a fluorescence confocal microscope. Immunoreactivity was evaluated by two investigators (LR and AG) and discordant cases were subsequently discussed and agreed upon. Thermo Scientific DAPI Nuclear Counterstains was used for nuclear staining.
3
NETosis Marker Expression in FFPE Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescence staining to observe the expression of NETosis markers was performed on formalin-fixed paraffin-embedded (FFPE) tissues utilizing the following primary antibodies: rabbit polyclonal anti-Elane (Atlas) at the dilution of 1:50 (citrate based pH 9.0 epitope retrieval solution); chicken polyclonal anti-Histone H2B (Abcam) at the dilution of 1:500 (citrate based pH 9.0 epitope retrieval solution) rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) (Abcam) at the dilution of 1:50 (citrate based pH 6.0 epitope retrieval solution). The following were used as secondary antibodies: CyTM2 Affinipure Donkey Anti-rabbit (Jackson Immuno Research) at the dilution of 1:400, CYTM3 Affinipure Donkey Anti-chicken (Jackson Immuno Research) at the dilution of 1:400. The colorations were read and interpreted by using a fluorescence confocal microscope. Immunoreactivity was evaluated by two investigators (LR and AG) and discordant cases were subsequently discussed and agreed upon. Thermo Scientific DAPI Nuclear Counterstains was used for nuclear staining.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!