8 epi pgf2a kit

Morning urine of all subjects was collected from 8–10 am after a 12-h fast (for footballers, 72 h after the last football training). The levels of 8-OHdG were measured by a competitive enzyme-linked immunosorbent assay (ELISA) using a commercial kit (8-OHdG kit, Abbexa, United Kingdom; Catalog No: abx150312). F_2a-IP levels were also measured by a competitive ELISA using a commercial kit (8-epi PGF2a kit, Abbexa, United Kingdom; Catalog No: abx150311). Then, the obtained values were standardized based on the amount of creatinine in urine. Creatine was measured using a spectrophotometry test kit (Pars Azmoon, Tehran, Iran).

Morning urine samples (8–10 AM) were obtained from all of the fasted participants (for athletes the three days after the last football training), and all subjects were instructed to avoid caffeine a day before urine sampling. Enzyme-linked immunosorbent assay (ELISA) was used to estimate urinary levels of 8-OHdG (8-OHdG kit, Abbexa, United Kingdom) with the catalog number of abx150312, and F_2a-IP (8-epi PGF_2a kit, Abbexa, United Kingdom) with the catalog number of abx150311. Then, F_2a-IP and 8-OHdG levels were reported based on the amount of urinary creatinine. Based on the kit instructions, the creatinine measurement was applied by spectrophotometry assay (Pars Azmoon, Tehran, Iran).

After a 12-h fasting period, morning urine samples were collected from 8–10 (a.m.) from all participants (for football players, 3 days after the last football training). Urinary levels of 8-OHdG and F_2a-IP were determined by an enzyme-linked immunosorbent assay (ELISA) using commercial kits (the 8-OHdG kit, abx150312, Abbexa, United Kingdom, and the 8-epi PGF_2a kit, abx150311, Abbexa, United Kingdom) according to the manufacturer's instructions. Values for 8-OHdG and F_2a-IP were normalized by creatinine measured in urine (spectrophotometry creatinine test kit, Pars Azmoon, Tehran, Iran).