Pci neo mova

Female WT C57BL/6, BALB/c, and NOD-SCID IL2Rg^null (NSG) mice (6–8 weeks old) were purchased from Shanghai Jie Si Jie Laboratory or Beijing Biocytogen and allowed to acclimatise for 1–2 weeks before experimentation. All animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Shanghai General hospital affiliated with Shanghai Jiao Tong University School of Medicine (2019-A012-01).
The cell lines B16-F0, B16-F10, 4T1, and MC38 were obtained from the American Type Culture Collection (ATCC). B16-GMCSF cells were generated by retroviral-mediated gene transfer, following the previously described^{57 (link)}. B16-GMCSF-OVA was ovalbumin (OVA)-transfected clone derived from B16-GMCSF cells which were transfected with the plasmid pCI-neo-mOVA (Cat. 25099, Addgene). Cells were cultured using RPMI-1640 (Corning) with 10% fetal bovine serum (Corning) and 1% Pen/Strep (GIBCO). Cells were incubated in an incubator maintained at 37 °C and 5% CO₂.

Mouse melanoma cell B16, mouse colon adenocarcinoma cell MC38, and 293T cell lines were obtained from Institute of Basic Medicine, Chinese Academy of Medical Sciences (Beijing, China). B16‐OVA and MC38‐OVA were generated from B16 and MC38 respectively which were stably transfected with the plasmid pCI‐neo‐mOVA (Addgene). Mouse hybridoma cell B3Z was kindly gifted by Dr Nilabh Shastri (University of California, Berkeley, California, USA). All cell lines were cultured in PRMI 1640 (Gibco, Pittsburgh, PA, USA) or DMEM (Gibco, Pittsburgh, PA, USA) supplemented with 10% FBS, 0.1% streptomycin and 100 U mL⁻¹ penicillin at 37 °C in a humidified atmosphere with 5% CO₂. Natural product chemical monomers were purchased from selleck (Beijing, China). Other reagents and commercial assay kits used in this study were listed in Table S1, Supporting Information. The antibodies used in this study were listed in Tables S2 and S3, Supporting Information.