Gold 3 chloride trihydrate haucl4

Manufactured by Merck Group

Sourced in United States

Gold (III) chloride trihydrate (HAuCl4) is a compound used as a precursor in the synthesis of various gold-based materials. It is a yellow-orange crystalline solid that is soluble in water and other polar solvents. The compound is commonly used in the preparation of gold nanoparticles, catalysts, and other gold-containing materials for various scientific and industrial applications.

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Gold(III) chloride trihydrate (296 citations) HAuCl4 (225 citations) Gold chloride trihydrate (57 citations) Gold (III) chloride trihydrate (HAuCl4·3H2O) (39 citations) Gold(III) chloride (17 citations) Gold chloride (14 citations) Gold (III) chloride ( HAuCl4 ) (4 citations) Gold chloride trihydrate (HAuCl4, (2 citations)

15 protocols using gold 3 chloride trihydrate haucl4

Seedless Synthesis of Gold IGNs

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IGNs were synthesized with a biological buffer, HEPES, through a one-step and seedless mediated method that was previously described by our group. Briefly, 18 mL of ultrapure water at 18 MΩ was added to 12 mL of 270 mM HEPES buffer at pH 7.40 ± 0.2. Next, 300 μL of 20 mM chloroauric acid was added. The solution was then mixed by gentle inversion and reacted for 75 min at room temperature. Both IGN synthesis materials, gold(III) chloride trihydrate (HAuCl₄) and HEPES, were purchased from Sigma-Aldrich.

Ou Y.C., Wen X., Johnson C.A., Shae D., Ayala O.D., Webb J.A., Lin E.C., DeLapp R.C., Boyd K.L., Richmond A., Mahadevan-Jansen A., Rafat M., Wilson J.T., Balko J.M., Tantawy M.N., Vilgelm A.E, & Bardhan R. (2020). Multimodal Multiplexed Immunoimaging with Nanostars to Detect Multiple Immunomarkers and Monitor Response to Immunotherapies. ACS nano, 14(1), 651-663.

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Synthesis and Characterization of Hyaluronic Acid-Gold Nanocomposites

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Sodium hyaluronate (HA-200 kDa and 700 kDa) was a kind gift from Fidia Farmaceutici S.p.A (IT). d-(+)-glucose purity ≥ 99.5%, gold (III) chloride trihydrate (HAuCl₄) purity ≥ 99.9 % and sodium hydroxide (NaOH), were purchased from Sigma-Aldrich. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT reagent), Dulbecco’s modified eagle medium (DMEM F-12) high glucose, RPMI-1640 and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO). Ultrapure Milli-Q water was used (18.2 mΩ·cm at 25 °C, Millipore).

Sanfilippo V., Caruso V.C., Cucci L.M., Inturri R., Vaccaro S, & Satriano C. (2020). Hyaluronan-Metal Gold Nanoparticle Hybrids for Targeted Tumor Cell Therapy. International Journal of Molecular Sciences, 21(9), 3085.

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Synthesis of Multifunctional Nanoparticles

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All reagents were used as received from the suppliers without further purification. Tetraethylorthosilicate (TEOS), 3-aminopropyltriethoxysilane (APTS), polyvinylpyrrolidone (PVP) (Mw 40,000), silver nitrate (AgNO₃), L-ascorbic acid, Tween 20, tetrakis(hydroxymethyl)phosphonium chloride (THPC), gold (III) chloride trihydrate (HAuCl₄), and histamine dihydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethyl alcohol (EtOH) and aqueous ammonium hydroxide (NH₄OH) were purchased from Daejung (Siheung, South Korea). HCl and NaOH were purchased from Samchun (Pyeongtaek, South Korea). Phosphate-buffered saline (PBS; 20×) was purchased from Dyne Bio (Seongnam, South Korea). Ultrapure water (resistivity 18.2 MΩ×cm) was produced using a Millipore water purification system (EXL water purification, Vivagen Co., Ltd., Seongnam, South Korea).

Huynh K.H., Pham X.H., Hahm E., An J., Kim H.M., Jo A., Seong B., Kim Y.H., Son B.S., Kim J., Rho W.Y, & Jun B.H. (2020). Facile Histamine Detection by Surface-Enhanced Raman Scattering Using SiO2@Au@Ag Alloy Nanoparticles. International Journal of Molecular Sciences, 21(11), 4048.

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ITO-Based Electrochemical Sensor Fabrication

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An indium tin oxide (ITO) electrode was obtained from U.I.D. (Cheongju, South Korea). Gold(III) chloride trihydrate (HAuCl₄), poly(ethylene glycol) 200 (PEG 200), Triton X‐100, and phosphate‐buffered saline (PBS) were purchased from Sigma‐Aldrich (Saint Louis, Missouri, USA). Polydimethylsiloxane (PDMS) was obtained from Dow Corning Crop. (Midland, MI, USA). The solutions used in this study were prepared with deionized (DI) water, which was purified using a Millipore Milli‐Q Direct Water Purification System (EMD Millipore, MA, USA).

Koo K., Kim C., Kim H., Cho Y., Suhito I.R, & Kim T. (2023). Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions. Advanced Science, 10(9), 2207084.

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Stimuli-Responsive Nanogel for Targeted Doxorubicin Delivery

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Gold(III) chloride trihydrate (HAuCl₄), sodium citrate tribasic dihydrate, amine-terminated pNIPAm
(average M_n 2500), NIPAm, and NIPMAm were
obtained from Sigma-Aldrich. Sodium dodecyl sulfate (SDS) was obtained
from Merck. Ammonium persulfate (APS) and BIS were obtained from Loba
Chemie. Doxorubicin hydrochloride (Adriamycin) was procured from Pfizer.
HeLa cells were obtained from the National Centre for Cell Sciences,
Pune, India. Dulbecco’s modified Eagle medium (DMEM, high glucose),
Dulbecco’s phosphate-buffered saline, calcein AM, trypsin,
FBS, and MTT were procured from Thermo Fisher Scientific.

Deshpande S., Sharma S., Koul V, & Singh N. (2017). Core–Shell Nanoparticles as an Efficient, Sustained, and Triggered Drug-Delivery System. ACS Omega, 2(10), 6455-6463.

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Synthesis of Thermoresponsive Hydrogel Nanoparticles

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Gold(III) chloride trihydrate (HAuCl₄; Sigma-Aldrich, Oakville, ON, Canada, ≥99.9%), sodium citrate tribasic dihydrate (SCTD; Sigma-Aldrich, Oakville, ON, Canada, ≥99.9%), sodium dodecyl sulfate (SDS; Sigma-Aldrich, Oakville, ON, Canada, 95%), butenylamine hydrochloride (B-en-A; Sigma-Aldrich, Oakville, ON, Canada, 97%), N,N′-methylenebisacrylamide (BIS; Sigma-Aldrich, Oakville, ON, Canada, 99%), and potassium persulfate (KPS; Sigma-Aldrich, Oakville, ON, Canada, 99%) were used as received. N-isopropylacrylamide (NIPAM; Sigma-Aldrich, Oakville, ON, Canada, 97%) was purified by recrystallization in n-hexane prior to use. Nanopure water (18 MΩ) was used in all experiments. Glassware and magnetic stirrers used for the syntheses were cleaned with aqua regia and thoroughly rinsed with water.

Sepúlveda A., Picard-Lafond A., Marette A, & Boudreau D. (2021). Nucleation Points: The Forgotten Parameter in the Synthesis of Hydrogel-Coated Gold Nanoparticles. Polymers, 13(3), 373.

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Synthesis and Functionalization of Gold Nanoparticles

Cited 1 time

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Spherical AuNPs with 13-nm diameter were synthesized by boiling gold(III) chloride trihydrate (HAuCl₄, Sigma-Aldrich) in trisodium citrate solution following a published protocol.^{46 (link)} We synthesized 40-nm gold nanostars following a previously established protocol.⁴⁷ CpG ODN (5’-TCC ATG ACG TTC CTG ACG T-3’, phosphodiester (PO) backbone) with a disulfide bond and a spacer phosphoramidite-18 coupled at 3’-end was prepared by solid phase synthesis. Unconjugated spherical AuNPs with 50-nm diameter were purchased from Ted Pella, INC. ODN 2087 and 2088 were purchased from Invivogen. Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride, 11-(mercaptoundecyl)hexa(ethylene glycol) (MUHEG), iodine (I₂), potassium iodide (KI), sodium borohydride (NaBH₄), dithiothreitol, hydrochloric acid (HCl, 36–38%), and nitric acid (HNO₃, 70%) were purchased from Sigma-Aldrich. Blocker™ BSA (bovine serum albumin) in phosphate-buffered saline (PBS) (10X), fetal bovine serum (FBS), rabbit serum and Dulbecco’s PBS (DPBS) were purchased from Thermo Fisher Scientific Inc.

Yue J., Pallares R.M., Cole L.E., Coughlin E.E., Mirkin C.A., Lee A, & Odom T.W. (2018). Smaller CpG-conjugated Gold Nanoconstructs Achieve Higher Targeting Specificity of Immune Activation. ACS applied materials & interfaces, 10(26), 21920-21926.

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Exosome Isolation and Characterization

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We used the following materials and equipment: fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), ExoQuick-TC reagent (SBI, Los Angeles, CA, USA), phosphate-buffered saline (PBS; Biosesang, Gyeonggi-do, Korea), gold(III) chloride trihydrate (HAuCl₄; Sigma-Aldrich, St. Louis, MO, USA), deionized sterile water (DW; Bioneer, Daejeon, Korea), Macrosep Advance Centrifugal Device (30 kDa; Pall Corporation, Washington, NY, USA), Micro BCA^TM protein assay kit (Thermo Scientific, Waltham, MA, USA), Dulbecco’s modified eagle medium/high glucose (DMEM; HyClone, Logan, UT, USA), Roswell park MEMorial institute 1640 medium (RPMI; HyClone, Logan, UT, USA), Minimum essential media (MEM; Welgene, Gyeongsangbuk-do, Korea), penicillin-streptomycin (Gibco, Brooklyn, NY, USA), Dulbecco’s phosphate buffered saline(DPBS; Sigma-Aldrich, St. Louis, MO, USA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, Carlsbad, CA, USA), and 5-dodecanoylaminofluorescein (DAF; Invitrogen, Carlsbad, CA, USA).

Lee E.S., Cha B.S., Kim S, & Park K.S. (2021). Synthesis of Exosome-Based Fluorescent Gold Nanoclusters for Cellular Imaging Applications. International Journal of Molecular Sciences, 22(9), 4433.

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Tryptophan Quantification in Fruit Juices

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Tryptophan standard (T0254-5G, reagent grade, purity ≥ 98%) was obtained from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA). HPLC-grade methanol, acetonitrile, acetic acid, trimethoxymethylsilane, hydrochloric acid, sulfuric acid, phosphoric acid, and boric acid were purchased from Merck KGaA (Darmstadt, Germany); sodium hydroxide from Panreac Quimica SAU (Barcelona, Spain); graphite powder from Alfa Aesar (Kandel, Germany); and gold (III) chloride trihydrate (HAuCl₄) from Sigma Aldrich (St. Louis, MO, USA). All the reagents were of analytical grade and used without further purification. Purified water was obtained from the Milli-Q System (Billerica, MA, USA). Samples of fruit juices were purchased from local supermarkets (Mercadona and Supersol) in Puerto Real, Cadiz, Spain.

Takele A., Palacios-Santander J.M, & Palma M. (2022). A New Electrochemical Method to Determine Tryptophan in Fruit Juices: Development and Validation. Foods, 11(14), 2149.

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Fabrication of Silica Nanocomposite Biosensors

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Ethanol (99%), mEthanol (99%), hydrochloric acid (37% in water), ammonium hydroxide (25% in water), tetraethylorthosilicate (TEOS; 99%), triEthanolamine (TEOA; 99%), cetyltrimethylammonium bromide (CTAB; 99%), 3-APTES (99%), GA (25% in water), urease (from Canavalia ensiformis, type IX, powder, 50,000 to 100,000 U/g of solid), urea (99.9%), BSA (lyophilized powder) gold(III) chloride trihydrate (HauCl4), sodium citrate (NaCit), and HS-PEG5K-NH2 (HCl salt) were purchased from Sigma-Aldrich. PBSs were purchased from Thermo Fisher Scientific. Standard Grey V4 resin (FLGPGR04) for the 3D printing of rigid molds was purchased at FormLabs. SYLGARD 184 Silicone Elastomer was purchased from Ellsworth Adhesives Ibérica.

Hortelao A.C., Simó C., Guix M., Guallar-Garrido S., Julián E., Vilela D., Rejc L., Ramos-Cabrer P., Cossío U., Gómez-Vallejo V., Patiño T., Llop J, & Sánchez S. (2021). Swarming behavior and in vivo monitoring of enzymatic nanomotors within the bladder. Science robotics, 6(52).

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