Thy1.1 mice

C57/BL6 mice were obtained from Jackson Laboratories, bred in-house, and bred off-site at Charles River. Foxp3-RFP reporter mice (C57BL/6-Foxp3^tm1Flv/J), Foxp3-Ametrine reporter (C57BL/6.Foxp3^{flpo-mAmetrine}), and RAG1 knockout (C57BL/6-Rag1^tm1Mom/J) mice were bred in-house and off-site at Charles River. Foxp3^Cre-ERT2.GFP × Rosa26^{LSL.Td.Tomato} mice were bred in-house. Thy1.1 mice were obtained from Jackson Laboratories. Mice used in experiments were male and female between 6 and 10 wk old at the initiation of the study. All animal work and protocols in this study were approved by the University of Pittsburgh Institutional Animal Care and Use Committee, accredited by the Association for Assessment and Accreditation of Laboratory Animal Care.

CD4-Cre transgenic (TG) mice, OT-1 Tg mice, Thy1.1⁺ mice, and Thy1.2⁺ mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Pgam1^flox/flox mice were kindly provided by Drs. Hiroshi Kondoh and Takumi Mikawa (Kyoto University). In brief, the exon 2–4 of pgam1 was pinched by two loxP sequences, and FLP-flanked neo-cassette was inserted in the 5′ upstream region of exon 2 to generate the pgam1 targeting vector. Targeting vector was injected into C57BL/6 mouse embryonic stem cells. The founder mice were crossed with FLP transgenic mice to remove the neo-cassette gene to generate Pgam1-floxed mice. Pgam1-floxed mice were crossed with CD4-Cre TG mice to generate T-cell-specific Pgam1-deficient mice. Gene-manipulated mice with a C57BL/6 background were used in all experiments. These mice were purchased from Clea Japan, Inc. (Tokyo, Japan). Both male and female mice were used in the experiments. All mice were used at 8–12 weeks of age.
All of the animal experiments received approval from the Ehime University Administrative Panel for Animal Care. All animal care was conducted in accordance with the guidelines of Ehime University. All experiments using Lm-OVA were performed in accordance with the protocols approved by the Ehime University Institution Biosafety Committee.

Female C57BL/6J mice aged 6–12 weeks were used for all experiments. WT mice were purchased from Daehan Bio Link (Eumsung, Republic of Korea). Thy1.1 mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). All mice were housed under specific pathogen-free conditions, and all animal experiments were approved by the Sogang University Institutional Animal Care and Use Committee (approval no. IACUCSGU2019_09).