Total RNA was extracted from BMSCs and HUVECs using the TRIzol Up kit (TransGen Biotech, China). The PCR reaction system was prepared to a total reaction volume of 10 μl with 5 μl 2× PCR buffer, 0.2 μl upstream primers, 0.2 μl downstream primers, 2 μl complementary DNA (cDNA), and 2.6 μl double-distilled water. The reaction conditions were as follows: 95°C for five minutes, 95°C for ten seconds, and 60°C for 30 seconds (a total of 40 cycles).
Trizol up kit
Manufactured by Transgene
Sourced in China
The TRIzol Up kit is a reagent used for the isolation and purification of RNA from various biological samples. It is designed to facilitate the efficient extraction and recovery of high-quality RNA for downstream applications such as reverse transcription and real-time PCR analysis.
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Lab products found in correlation
2 protocols using trizol up kit
1
Total RNA Extraction and RT-qPCR Analysis
2
Rice Leaf RNA Extraction and qRT-PCR
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Rice leaf RNA was extracted from transgenic rice plants and wild-type FH7185 rice plants. Total RNA was extracted with a TriZol Up kit (TransGen Biotech, China) according to the manufacturer’s protocol. An RNA reverse-transcription kit with gDNA Remover (Toyobo, Japan) was used to generate cDNA for 10 min at 25 °C, 120 min at 37 °C and 5 min at 85 °C. qRT-PCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on an ABI Prism 7500 real-time PCR system.
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