Histological analysis was performed to examine the CVO and retinal damage. The rabbits were euthanized at day 28 post-photocoagulation. To euthanize the rabbits, euthanasia solution (Beuthanasia-D, 0.22 mg/kg, 50 mg/mL, Vetone, ID, USA) was injected into the rabbit intravenously. The eyeballs were harvested and fixed in Davidson’s fixative solution for 24 h. The sample was then cut into 5 mm pieces and embedded in paraffin. The sample was sectioned to a thickness of 4 µm using a Leica Autostainer XL (Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin (H&E). The H&E slides were observed using a Leica DM600 light microscope (Leica Biosystems, Nussloch, Germany) and the images were captured using a BF450C camera.
Dm600 light microscope
Manufactured by Leica
Sourced in Germany
The Leica DM600 is a light microscope designed for high-quality imaging and analysis. It features an LED illumination system, an advanced optical system, and a range of objective lenses to accommodate various sample types. The microscope is capable of delivering clear, detailed images for a variety of applications.
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Lab products found in correlation
4 protocols using dm600 light microscope
1
Histological Analysis of CVO and Retinal Damage
2
Histological Assessment of Retinal Damage
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Histological analysis was implemented to evaluate the degree of RVO, CVO, and retinal damage. The rabbits were euthanized at day 28 post-photocoagulation by intravenous injection of euthanasia solution (Beuthanasia-D, 0.22 mg/kg, 50 mg/mL, VetOne, Boise, ID, USA). The eyeballs were sectioned and fixed in Davidson’s fixative solution for 24 h. 100 μL of 4% formaldehyde buffer solution was intravitreally injected into the retina to prevent retinal detachment. The fixed sample was then cut into 5 mm specimens and embedded in paraffin. The embedded tissue was sectioned at a thickness of 4 μm using a Leica autostainer XL (Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin (H&E). The H&E slides were examined under microscope (Leica DM600 light microscope (Leica Biosystems, Nussloch, Germany) and the H&E images were captured using the BF450C camera.
3
Corneal Histology and TUNEL Assay
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Histological analysis and Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay were carried out to examine the structure of the cornea after treatment. Treated rabbits were euthanized at 48 h post treatment. The eyeballs were isolated and injected with 100 μL of 10% formalin. The harvested tissues were pre-fixed in Davidson fixation solution at room temperature for 24 h. The samples were transferred to 50% ethanol solution and incubated for 8 h. Next, the samples were placed in 70% ethanol solution for 24 h before embedding in paraffin. The tissues were sectioned to a thickness of 4 μm using a Leica Autostainer XL (Leica Biosystems) for H&E staining and TUNEL assay analysis. The TUNEL assay was performed using the in situ cell death detection kit (Promega, USA) as previously reported [64 ]. The corneal morphology was evaluated along with the microstructural change of the treated tissue on the H&E images. All slides were images with a Leica DM600 light microscope (Leica Biosystems). Digital images were captured using a BF450C camera for H&E and a BF360C camera for TUNEL (DM600; Leica Biosystems).
4
Rabbit Eye Tissue Fixation and Histology
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To euthanize the rabbits, euthanasia solution (Euthanasia, 0.22 mg/kg, 50 mg/mL, VetOne, ID, USA) was injected into the rabbit intravenously through the marginal ear vein. The eyeballs were harvested and fixed in Davidson's fixative solution for 24 h.
The sample was then cut into 5 mm pieces and embedded in paraffin. The sample was sectioned to a thickness of 4 µm using a Leica Autostainer XL (Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin (H&E). The H&E slides were observed using a Leica DM600 light microscope (Leica Biosystems, Nussloch, Germany) and the images were captured using a BF450C camera.
The sample was then cut into 5 mm pieces and embedded in paraffin. The sample was sectioned to a thickness of 4 µm using a Leica Autostainer XL (Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin (H&E). The H&E slides were observed using a Leica DM600 light microscope (Leica Biosystems, Nussloch, Germany) and the images were captured using a BF450C camera.
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