Cell counting kit 8 (cck8)

Manufactured by Selleck Chemicals

Sourced in United States, China

The Cell Counting Kit-8 is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes a water-soluble tetrazolium salt that is reduced by cellular dehydrogenase activities, producing a colored formazan dye. The amount of the formazan dye generated is directly proportional to the number of living cells.

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104 protocols using cell counting kit 8 (cck8)

Cell Growth Quantification with CCK-8 Assay

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CCK-8 assay was performed to mesure the growth rate of cells using Cell Counting Kit-8 (Selleck Chemicals), according to the manufacturer's instructions.

Mu L., Guan B., Tian J., Li X., Long Q., Wang M., Wang W., She J., Li X., Wu D, & Du Y. (2020). MicroRNA-218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2. Oncology Reports, 44(5), 1961-1970.

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Cell Proliferation Assay Using CCK8

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1000 cells per well were plated in 96-well plate and cultured for indicated days. Cell proliferation was evaluated using the Cell-Counting Kit 8 (CCK8, Selleck).

Zhang Y., Zhu Y., Chen Y., Wang Y., Liu B., Pan Y., Liao X., Pan J., Gao H., Yang W, & Yu G. (2024). Nuclear translocation of cleaved PCDH9 impairs gastric cancer metastasis by downregulating CDH2 expression. iScience, 27(2), 109011.

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Cell Viability and Apoptosis Evaluation

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Cells (1×10 ⁴) were placed in 96-well plates and then subject to specific
chemical treatments. After a 24‒72 h incubation period, cell viability was evaluated using
a Cell Counting Kit-8 (Selleck, Houston, USA), and detection was facilitated with the
Synergy H4 system (BioTek, Winooski, USA). An Annexin V 633 Apoptosis Detection kit
(Dojindo, Tokyo, Japan) and a FACS system (Beckman Coulter, Pasadena, USA) were used to
assess cell apoptosis.

Fei Q., Jin K., Shi S., Li T., Guo D., Lin M., Yu X., Wu W, & Ye L. (2024). Suppression of pancreatic cancer proliferation through TXNIP-mediated inhibition of the MAPK signaling pathway: TXNIP inhibits pancreatic cancer via the MAPK pathway. Acta Biochimica et Biophysica Sinica, 56(4), 513-524.

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Evaluating Compound Cytotoxicity on A549 Cells

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Cells viability was evaluated by Cell Counting Kit-8 (CCK-8; Selleck Chemicals, China). Briefly, A549 cells were digested and plated into each well of a 96-well plate after adjusting the cell suspension to the appropriate concentration of 1×10³ cells per 100 μL. The next day, the culture medium was discarded and the cells were exposed to Triptolide, Perillyl alcohol and α-Terpineol at different concentrations for 0 h, 24 h, 48 h, 72 h, 96 h and 120 h, 10 μL of CCK-8 (Bimake, United States) was added in the plates and cultured at 37 °C for 2 h. Absorbance at 450 nm was measured using spectrophotometer. The experiments were carried out in triplicate.

Zhang X., Wang K., Dai H., Cai J., Liu Y., Yin C., Wu J., Li X., Wu G., Lu A., Liu Q, & Guan D. (2022). Quantification of promoting efficiency and reducing toxicity of Traditional Chinese Medicine: A case study of the combination of Tripterygium wilfordii hook. f. and Lysimachia christinae hance in the treatment of lung cancer. Frontiers in Pharmacology, 13, 1018273.

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Cell Viability Measurement by CCK-8 Assay

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Cells were seeded into 96-well plates at 2,000 cells/well and cultured for 0, 24, 48, or 72 hours. Cell viability was measured using a Cell Counting Kit-8 assay (#B34302, Selleck, USA) according to the manufacturer’s instructions. The fluorescence in each plate was measured using a spectrophotometer at an emission wavelength of 450 nm (Beckman, USA).

Tao Q., Liu N., Wu J., Chen J., Chen X, & Peng C. (2024). Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors. Journal for Immunotherapy of Cancer, 12(3), e008554.

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Gefitinib Sensitivity Assay in NSCLC

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Cell proliferation was measured by CCK8 assay (cell counting kit-8, Selleck, Shanghai, China). PC9 cells and PC9/GR cells transfected with si-LINC00665 or si-NC were plated in 96-well plates at a density of 3 × 10³/well and incubated overnight. Subsequently, the cells were exposed to different concentrations of gefitinib (AstraZeneca, London, England, UK) for 72 h. Then, 10 μL of CCK8 was added into each well and incubated for 2 h. The optical density was measured at 450 nm by an enzyme-labeled instrument. All experiments were repeated three times independently.
In the colony-formation assay, a total of 800 si-LINC00665 or si-NC PC9/GR cells were placed in 6-well plates maintaining in media containing 10% FBS and exposed to gefitinib for 24 h. Then, the drugs were washed away and the medium was replaced every 4 days. After 2 weeks, the colonies were fixed with methanol and stained with a 0.1% crystal violet (Sigma, St. Louis, MO, USA). Visible colonies were counted. Each experiment was performed in triplicate.

Liu X., Lu X., Zhen F., Jin S., Yu T., Zhu Q., Wang W., Xu K., Yao J, & Guo R. (2019). LINC00665 Induces Acquired Resistance to Gefitinib through Recruiting EZH2 and Activating PI3K/AKT Pathway in NSCLC. Molecular Therapy. Nucleic Acids, 16, 155-161.

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Cell Viability Assessment of HeLa Cells

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Cell counting kit-8 (Selleck Chemicals, Houston, Texas, USA) was used in accordance with the manufacturer’s instructions. Hela cells were seeded in 96-well plates (1000 cells/well) and cultured at 37 °C in an incubator. A total of 10 μL of CCK8 solution was added to each well after 24, 48, 72, 96 and 120 h, respectively, and incubated for 2 h. Relative cell density was determined at a wavelength of 450 nm using the Biotek Synergy HTX (BioTek Instruments, VT, USA).

Sun Y., Li R., Nong B., Songyang Z., Wang X., Ma W, & Zhou Q. (2023). A Comprehensive Pan-Cancer Analysis of the Potential Biological Functions and Prognosis Values of RICTOR. Genes, 14(6), 1280.

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Cell Viability Measurement by CCK-8 Assay

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Cytotoxicity Assay of 5-FU

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SGC7901 and BGC823 cells were added in 96-well plates in triplicates and challenged with increasing concentrations of 5-FU (dissolved by CM and normal medium, respectively) for 72 h. Then, the cell survival was detected using Cell Counting Kit-8 regents (Selleck, USA).

Yang Y., Ma Y., Yan S., Wang P., Hu J., Chen S., Zhu J., Wang J., Chen G, & Liu Y. (2021). CAF promotes chemoresistance through NRP2 in gastric cancer. Gastric Cancer, 25(3), 503-514.

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Cell Viability Quantification via CCK-8

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Cell viability was quantified using the Cell Counting Kit-8 assay(B34304, Selleckchem). Cells were seeded into 96-well plates at a density of 3 × 10³ cells/well and were cultured in a humidified atmosphere with 5% CO₂ at 37 °C. Subsequently, 10 μl CCK-8 reagent was added to each well. The absorbance was measured at 450 nm after 2 h. The CCK-8 assay was performed every 24 h for 4 consecutive days.

Huang X.D., Du L., Cheng X.C., Lu Y.X., Liu Q.W., Wang Y.W., Liao Y.J., Lin D.D, & Xiao F.J. (2024). OTUB1/NDUFS2 axis promotes pancreatic tumorigenesis through protecting against mitochondrial cell death. Cell Death Discovery, 10, 190.

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