Curcumin, copper(II) sulfate pentahydrate (CuSO4·5H2O), zinc(II) chloride (ZnCl2) and iron(III) nitrate nonahydrate (Fe(NO3)3·9H2O) were purchased from Merck (Germany). Solvents with highest purity were bought from Merck (Germany) and used without further purifications. The elemental analyses (carbon, hydrogen and nitrogen) of complexes were obtained from a Carlo ERBA Model EA 1108 analyzer. The metal content of the complexes was determined by atomic absorption analysis on a Varian Spectra AA-220 equipment. Fourier transform infrared (FT-IR) spectroscopy was performed using a FT-IR Spectrometer Bruker Tensor 27 as KBr disks. Fresh stock solution of metal-CUR complexes (Cu-CUR, Zn-CUR, Fe-CUR) and free-CUR were prepared in dimethyl sulfoxide (DMSO) at the concentration of 1 mg/ml. N-Acyl-homoserine lactone (C6-HSL) (Sigma-Aldrich) was used at 20 µM in C. violaceum CV026 biosensor bioassay.
Model ea 1108
Manufactured by Carlo Erba
The EA 1108 is a CHNS/O elemental analyzer manufactured by Carlo Erba. It is designed to determine the elemental composition of organic and inorganic samples, including the percentage of carbon, hydrogen, nitrogen, sulfur, and oxygen present.
Automatically generated - may contain errors
Lab products found in correlation
Curcumin, by Merck Group (1 mentions)
Copper 2 sulfate pentahydrate, by Merck Group (1 mentions)
Iron 3 nitrate nonahydrate, by Merck Group (1 mentions)
Zinc 2 chloride, by Merck Group (1 mentions)
N acyl homoserine lactone c6 hsl, by Merck Group (1 mentions)
Sunrise absorbance reader, by Tecan (1 mentions)
E line spectrometer, by Agilent Technologies (1 mentions)
Ch3cn, by Honeywell (1 mentions)
Ls 45 fluorescence spectrometer, by PerkinElmer (1 mentions)
Avance 300 mhz, by Bruker (1 mentions)
4 protocols using model ea 1108
1
Synthesis and Characterization of Metal-Curcumin Complexes
2
Ash Content and Elemental Analysis of PDS
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The approximate analysis of PDS biomass was accomplished according to ASTM specifications. The production of ash was calculated at 550 • C for 4 h in a furnace. Carbon, hydrogen, nitrogen, and oxygen elemental compositions of PDS were determined using an elemental analyzer Model EA 1108 (Carlo Erba Instruments) and ASTM D3176 standard techniques (Hai et al., 2021) .
3
Comprehensive Analytical Characterization of Nanomaterials
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NMR spectra were obtained on a Bruker spectrometer with CDCl3 as solvent and TMS as an internal standard. Chemical shifts (δ) are given in ppm and coupling constants (J) are given in Hz. FT-IR spectra were recorded with KBr pellets by a Magna-IR, spectrometer 550 Nicolet. CHN compositions were measured by Carlo ERBA Model EA 1108 analyzer. Powder X-ray diffraction (XRD) was carried out on a Philips diffractometer of X’pert Company with monochromatized Cu Kα radiation (λ = 1.5406 Å). Microscopic morphology of products was visualized by SEM (MIRA3). The thermogravimetric analysis (TGA) curves are recorded using a V5.1A DUPONT 2000. The mass spectra were recorded on a Joel D-30 instrument at an ionization potential of 70 eV. The magnetic property of magnetite nanoparticle has been measured with a vibrating sample magnetometer (VSM) (Meghnatis Daghigh Kavir Co.; Kashan Kavir; Iran) at room temperature.
4
Characterization of Novel Compounds
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CQDP, calf thymus DNA (CT-DNA), buffers and solvents were used as received from Sigma-Aldrich Co. All other commercial reagents were also used without further purification. The NMR spectra of the compounds were recorded in CH2Cl2-d2 and DMSO-d6 solution in Bruker AVANCE 300 MHz or 500 MHz spectrometers. IR spectra were recorded with a Perkin Elmer Series 100 spectrometer in the 4000–400 cm−1 region, ultraviolet-visible (UV-Vis) spectra on a HP 8453 diode array spectrometer and fluorescence spectra on a Perkin Elmer LS45 fluorescence spectrometer. C, H and N analyses were performed using a Carlo Erba Model EA1108 elemental analyzer. ESI (+) MS spectra were obtained by direct infusion in a QTOF Bruker Impact II Mass Spectrometer in positive ion mode, utilizing CH3CN (LC/MS grade from Honeywell; B&J Brand) as the solvent. Electron Paramagnetic Resonance (EPR) spectra were performed on a Varian E-Line spectrometer. In the β-hematin formation inhibition assay plates were centrifuged using a Thermo Scientific IEC CL 30 centrifuge and measured in a Tecan Sunrise Absorbance Reader at 405 nm.
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