Phospho jak2 antibody

Manufactured by Cell Signaling Technology

The Phospho-JAK2 antibody is a laboratory reagent used to detect the phosphorylated form of the Janus Kinase 2 (JAK2) protein. JAK2 is a critical component of cellular signaling pathways and its phosphorylation is an important indicator of pathway activation. This antibody can be used in various analytical techniques, such as Western blotting, to study the regulation and function of JAK2 in biological systems.

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Phospho-JAK2 (45 citations) JAK2 antibody (3 citations)

2 protocols using phospho jak2 antibody

Comprehensive Immune Marker Antibody Panel

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PBRM1 antibody (A301-591A) was from Bethyl Laboratories. Phospho-STAT1 antibody (clone ST1P-11A5; Tyr701; 33-3400), human CD3 antibody (clone F7.2.38; MA5-12577), human CD45RO antibody (clone UCHL1; MA5-11532), human CD4 antibody (clone 4B12; MS1528S0), and human CD8 antibody (clone C8/144B; MS457S0) were from ThermoFisher Scientific. Mouse CD3 antibody (D4V8L; 99940), mouse CD8 antibody(D4W22; 98941), mouse CD4 antibody (D7D2Z; 25229), human PD-L1 antibody (E1L3N; 13684), mouse PD-L1 antibody (D5V3B; 64988), mouse PD-1 antibody (D7D5W; 84651), PBRM1 antibody (D3F7O, 91894), JAK1 antibody (6G4, 3344), JAK2 antibody (D2E12, 3230), Phospho-JAK1 antibody (D7N4Z, Tyr1034/1035, 74129), Phospho-JAK2 antibody (C80C3, Tyr1007/1008, 3776), STAT1 antibody (D1K9Y, 14994), Phospho-STAT1 antibody (58D6, Tyr701, 9167), Phospho-STAT1 antibody (D3B7, Ser727, 8826), IRF1 antibody (D5E4, 8478), and BRG1 antibody (E9O6E; 52251) were from Cell Signaling Technology. IFNGR1 antibody (112802) was from BioLegend. IFNGR2 antibody (Cat No. GTX 64548) was from GeneTex. β-actin antibody (A1978) was from Sigma. Human IFNγ (285-IF-100) and mouse IFNγ (8234-MB-010) were from R&D Systems.

Liu X.D., Kong W., Peterson C.B., McGrail D.J., Hoang A., Zhang X., Lam T., Pilie P.G., Zhu H., Beckermann K.E., Haake S.M., Isgandrova S., Martinez-Moczygemba M., Sahni N., Tannir N.M., Lin S.Y., Rathmell W.K, & Jonasch E. (2020). PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma. Nature Communications, 11, 2135.

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Western Blot Antibody Panel Validation

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The antibodies for western blotting were as follows: anti-RORC (1:250, SAB1100574) was from Sigma, and JAK2 (Product # 3230), and phospho-JAK2 antibody (Product # 3776), STAT3 (Product # 12640) and phospho-STAT3 antibody (Product # 9145), and GAPDH antibody (Product # 5174) were purchased from Cell Signaling Technology. Secondary antibodies were bought from ZSGB-BIO (Beijing, China). All experiments were conducted in triplicate.

Chen Y., Liu Z., Yu Q., Qu X, & Liu H. (2023). Integrating network pharmacology and experimental verification to explore the mechanism of Tripterygium wilfordii in ankylosing spondylitis. Medicine, 102(50), e36580.

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