Y26732

Manufactured by Selleck Chemicals

Sourced in United States

The Y26732 is a laboratory equipment product designed for general laboratory use. It serves as a tool for various scientific and research applications. The core function of this product is to facilitate common laboratory tasks and procedures. No further details or interpretation on the intended use are provided.

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Lab products found in correlation

2 protocols using y26732

Breast Cancer Cell Culture and Viability Assays

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Phenol-red-free DMEMF/12 (PRF-DMEM/F12), charcoal-stripped FBS (CS-FBS), penicillin–streptomycin, GlutaMAX-I, insulin-transferrin-selenium (ITS), Dulbecco’s phosphate-buffered saline (DPBS), TrypLE express, paraformaldehyde (PFA), 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI), and Cell Tracker™ Green 5-chloromethylfluorescein diacetate (CMFDA) were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Dimethyl sulphoxide (DMSO), Triton X-100, and estradiol-17β (estrogen) were purchased from Sigma-Aldrich, Castle Hill NSW 2154, Australia. The Real Time-Glo™ MT Cell Viability Assay was purchased from Promega, Alexandria NSW 2015, Australia. Salinomycin, panobinostat, fulvestrant, tamoxifen, and Y26732 were purchased from Selleck Chem, Houston, TX 77014 USA.

Churchill M.L., Holdsworth-Carson S.J., Cowley K.J., Luu J., Simpson K.J., Healey M., Rogers P.A, & Donoghue J.F. (2023). Using a Quantitative High-Throughput Screening Platform to Identify Molecular Targets and Compounds as Repurposing Candidates for Endometriosis. Biomolecules, 13(6), 965.

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Lipid Signaling Pathway Characterization

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Oleoyl (18:1)-LPA was purchased from Avianti Polar Lipids (Alabaster, AL; # 857128X). The LPAR1/3 inhibitor Ki16425 (#10012659) was purchased from Cayman Chemical Company (Ann Arbor, MI) and the selective ROCK1/2 inhibitor Y26732 was from Selleckchem (#S6390). See Table S1 for a list of the antibodies, primers, and short hairpin RNAs used in this study. Fluorescein isothiocyanate (FITC)- and horseradish peroxidase-conjugated donkey anti-mouse and anti-rabbit secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). Enhanced chemiluminescence reagents were from Thermo Scientific Pierce Protein Biology Products (Rockford, IL).

DOUTT S.W., LONGO J.F, & CARROLL S.L. (2022). LPAR1 and aberrantly expressed LPAR3 differentially promote the migration and proliferation of malignant peripheral nerve sheath tumor cells. Glia, 71(3), 742-757.

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