Combined total plant RNA and small RNA extractions were performed using a single leaf that was snap-frozen in liquid nitrogen, homogenized, divided into two approximately 50 mg aliquots and processed with a Thermo Scientific GeneJET Plant RNA Purification Mini Kit (Thermo Scientific, Waltham, MA, USA) and mirPremier microRNA Isolation Kit (Sigma-Aldrich, St. Louis, MI, USA), respectively, following manufacturers’ recommendations. The quantification and quality control of the RNA extracts were performed using a Nanodrop 1000 UV-Vis spectrophotometer and Qubit HS RNA and IQ assays (Invitrogen, Carlsbad, CA, USA).
Qubit hs rna and iq assays
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Qubit HS RNA and IQ assays are quantification tools for measuring the concentration of RNA samples. They provide accurate and sensitive measurements across a wide dynamic range, enabling reliable quantification of RNA samples prior to downstream applications such as reverse transcription and sequencing.
Automatically generated - may contain errors
Lab products found in correlation
Genejet plant rna purification mini kit, by Thermo Fisher Scientific (2 mentions)
Nanodrop 1000 uv vis spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Mirpremier microrna isolation kit, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Tri reagent, by Merck Group (1 mentions)
2 protocols using qubit hs rna and iq assays
1
Rapid RNA Extraction from Plant Leaves
2
RNA Extraction from Plants and Aphids
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total plant RNA extraction was performed from 50 mg of plant fresh material snap-frozen in liquid nitrogen using the Thermo Scientific GeneJET Plant RNA Purification Mini Kit (Thermo Scientific) following the manufacturers’ recommendations. Total aphid RNA extraction was performed using TRI Reagent (Sigma Aldrich, St. Louis, MO, USA) or TRIzol (Invitrogen, Carlsbad, CA, USA) kits from a single aphid individual. Quantification and quality control of RNA extracts were performed using Nanodrop 1000 UV-Vis spectrophotometer and Qubit HS RNA and IQ assays (Invitrogen).
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